Volume 12, Issue 3, August 2015, Pages 542–551
Majida Lahrouni1, Khalid Oufdou2, Fatima El Khalloufi3, Eloisa Pajuelo4, and Brahim Oudra5
1 Laboratory of Biology and Biotechnology of Microorganisms, Environmental Microbiology and Toxicology Unit, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco
2 Laboratory of Biology and Biotechnology of Microorganisms, Environmental Microbiology and Toxicology Unit, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco
3 Laboratory of Biology and Biotechnology of Microorganisms, Environmental Microbiology and Toxicology Unit, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco
4 Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, Spain
5 Laboratory of Biology and Biotechnology of Microorganisms, Environmental Microbiology and Toxicology Unit, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco
Original language: English
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The occurrence of toxic freshwater blooms of cyanobacteria has been frequently reported during the last 15 years in Lalla Takerkoust lake (35 km southwestern of Marrakech city) In this study, it has been confirmed that the collected cyanobacteria bloom could produce different variants of microcystins at high concentration of 11.5 mg equiv. MC-LR g-1DW of the cyanobacteria cells. In order to study the effect of microcystins on faba bean seedling cultured in vitro, the crude aqueous extract of the toxic bloom was prepared and sterilized by filtration, and then it was supplemented to BNM medium at different concentrations. After 10 days of in vitro seedlings growing in BNM medium, plants fresh and dry weights were determined, plants shoot and root length was measured and then the roots were subjected to histological microscope observation of root hair, root tip and root cortical cells. The results revealed that microcystins exposure induced a decreasing of seedling growth and biomass accumulation in a concentration dependent manner. In addition, seedling young roots exhibited a brownish aspect, necrosis and tissue lysis. At a microcystin's concentration of 40
Author Keywords: Cyanobactria, Faba bean, microcystins, root hair, root tip, cortical root cells, irrigation.
Majida Lahrouni1, Khalid Oufdou2, Fatima El Khalloufi3, Eloisa Pajuelo4, and Brahim Oudra5
1 Laboratory of Biology and Biotechnology of Microorganisms, Environmental Microbiology and Toxicology Unit, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco
2 Laboratory of Biology and Biotechnology of Microorganisms, Environmental Microbiology and Toxicology Unit, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco
3 Laboratory of Biology and Biotechnology of Microorganisms, Environmental Microbiology and Toxicology Unit, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco
4 Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, Spain
5 Laboratory of Biology and Biotechnology of Microorganisms, Environmental Microbiology and Toxicology Unit, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco
Original language: English
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The occurrence of toxic freshwater blooms of cyanobacteria has been frequently reported during the last 15 years in Lalla Takerkoust lake (35 km southwestern of Marrakech city) In this study, it has been confirmed that the collected cyanobacteria bloom could produce different variants of microcystins at high concentration of 11.5 mg equiv. MC-LR g-1DW of the cyanobacteria cells. In order to study the effect of microcystins on faba bean seedling cultured in vitro, the crude aqueous extract of the toxic bloom was prepared and sterilized by filtration, and then it was supplemented to BNM medium at different concentrations. After 10 days of in vitro seedlings growing in BNM medium, plants fresh and dry weights were determined, plants shoot and root length was measured and then the roots were subjected to histological microscope observation of root hair, root tip and root cortical cells. The results revealed that microcystins exposure induced a decreasing of seedling growth and biomass accumulation in a concentration dependent manner. In addition, seedling young roots exhibited a brownish aspect, necrosis and tissue lysis. At a microcystin's concentration of 40
Author Keywords: Cyanobactria, Faba bean, microcystins, root hair, root tip, cortical root cells, irrigation.
How to Cite this Article
Majida Lahrouni, Khalid Oufdou, Fatima El Khalloufi, Eloisa Pajuelo, and Brahim Oudra, “Impact of cyanobacterial toxins (microcystins) on growth and root development of in vitro Vicia faba cultures,” International Journal of Innovation and Applied Studies, vol. 12, no. 3, pp. 542–551, August 2015.
