[ Micropropagation in vitro et régénération de niébé (Vigna unguiculata (L.) Walp) à partir des nœuds cotylédonaires ]
Volume 21, Issue 2, September 2017, Pages 247–253
Chahinaz Chekroun1 and Moulay Belkhodja2
1 Laboratoire de Physiologie Végétale, Département de Biologie, Faculté des Sciences de la Nature et de la Vie, Université d’Oran 1 Ahmed Ben Bella, Oran, Algeria
2 Laboratoire de Biotoxicologie Expérimentale, Biodépollution et de Phytoremediation BTEBDPR, Faculté des Sciences, Université Es-Senia - Oran, Algeria
Original language: French
Copyright © 2017 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Vigna unguiculata (L.) Walp plant was efficiently regenerated using cotyledonary nodes as explant. The explants were excised from 3 days old seedling grown in vitro on MS medium containing 3 mg/l BAP. They were cultured on MS medium containing BAP (2,5 mg/l) combinated with different dose of NAA (0, 0,1 and 0,5 mg/l) for shoot induction. Regeneration frequency varied with growth regulator combinations in the medium. A maximum number of 9,6 shoots per explant was recorded on MS medium containing 2.5 mg/l BAP. Increase in the ANA concentration of the culture medium decreased the number of shoots. Regenerated shoots were elongated on MS medium containing 1,25 mg/l BAP. The elongated shoots were rooted on MS rooting medium without hormone. The rooted plants were transferred to soil with a survival rate of 80%.
Author Keywords: MS medium, shoot induction, regeneration frequency, rooting.
Volume 21, Issue 2, September 2017, Pages 247–253
Chahinaz Chekroun1 and Moulay Belkhodja2
1 Laboratoire de Physiologie Végétale, Département de Biologie, Faculté des Sciences de la Nature et de la Vie, Université d’Oran 1 Ahmed Ben Bella, Oran, Algeria
2 Laboratoire de Biotoxicologie Expérimentale, Biodépollution et de Phytoremediation BTEBDPR, Faculté des Sciences, Université Es-Senia - Oran, Algeria
Original language: French
Copyright © 2017 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Vigna unguiculata (L.) Walp plant was efficiently regenerated using cotyledonary nodes as explant. The explants were excised from 3 days old seedling grown in vitro on MS medium containing 3 mg/l BAP. They were cultured on MS medium containing BAP (2,5 mg/l) combinated with different dose of NAA (0, 0,1 and 0,5 mg/l) for shoot induction. Regeneration frequency varied with growth regulator combinations in the medium. A maximum number of 9,6 shoots per explant was recorded on MS medium containing 2.5 mg/l BAP. Increase in the ANA concentration of the culture medium decreased the number of shoots. Regenerated shoots were elongated on MS medium containing 1,25 mg/l BAP. The elongated shoots were rooted on MS rooting medium without hormone. The rooted plants were transferred to soil with a survival rate of 80%.
Author Keywords: MS medium, shoot induction, regeneration frequency, rooting.
Abstract: (french)
Vigna unguiculata (L.) Walp est efficacement régénérée à partir des nœuds cotylédonaires. Les explants sont prélevés à partir de graines germées de trois jours sur le milieu MS additionné de 3 mg/l BAP. Les explants sont cultivés sur le milieu MS contenant du BAP (2,5 mg/l) combiné avec différentes concentrations de NAA (0, 0,1 et 0,5 mg/l) pour l'induction des pousses. La fréquence de régénération varie avec la combinaison hormonale dans le milieu. Un nombre maximal de 9,6 pousses par explant est obtenu sur le milieu MS contenant 2,5 mg/l de BAP. L'augmentation de la concentration d'ANA du milieu de culture induit une diminution du nombre de pousses. L’élongation des pousses régénérées est obtenue par culture des explants sur le milieu d’élongation où la concentration du BAP est réduite de moitié (milieu MS additionné de 1,25 mg/l BAP). Les pousses sont enracinées sur un milieu d'enracinement (MS ½ macro sans hormone). Les plantes enracinées sont transférées dans le sol avec un taux de survie de 80%.
Author Keywords: milieu MS, induction des pousses, fréquence de régénération, enracinement.
How to Cite this Article
Chahinaz Chekroun and Moulay Belkhodja, “In vitro micropropagation and plants regeneration of Cowpea (Vigna unguiculata (L.) Walp) from cotyledonary node,” International Journal of Innovation and Applied Studies, vol. 21, no. 2, pp. 247–253, September 2017.
