[ Caractérisation phénotypique des entérobactéries antibiorésistantes isolées des voies urogénitales et sensibilité aux extraits des alicaments (Allium sativum, Allium schoenoprasum, Allium cepa et Allium porrum) à Kisangani (Province de la Tshopo, RD Congo) ]
Volume 29, Issue 4, July 2020, Pages 1015–1026
O. Lokonga1 and M. Dasangba2
1 Departement of Biotechnologics Sciences, Faculty of Sciences, B.P. 2012, University of Kisangani, RD Congo
2 Département des sciences Biotechnologiques, Faculté des sciences, B.P. 2012, Université de Kisangani, RD Congo
Original language: French
Copyright © 2020 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The objective of this work is to determine the phenotypic characterization of enterobacteria with a view to identifying them, extracting aromatic oils, and preparing ethereal, chloroformic extracts of foods, in order to test the antibacterial activity of four foods, among other Allium sativum, Allium schoenoprasum, Allium cepa and Allium porrum.The aqueous, ethereal and chloroform extracts were obtained by hydro-distillation and by the extraction method using ether and chloroform respectively.As a result, the strains observed are Gram negative bacteria; their morphological and biochemical characterization made it possible to identify three germs including Citrobacter sp, Proteus mirabilis and Escherichia coli.The study of the sensitivity of these strains to hydro-distillation, to the ethereal and chloroform extract of Allium sativum, Allium schoenoprasum, Allium cepa and Allium porum by the method of diffusion in agar medium led to the following results: strains S8, S13 and S16 inhibited on hydro distillation (8; 15; 16mm of inhibition diameters); the ethereal extract of these same drugs inhibited the S14 strains for garlic (with 8mm inhibition diameter; S13 and S16 for chives (with 10mm inhibition diameter) and finally, the S16 strain for onion (with 16mm inhibition diameter), on the other hand, the chloroformic extract presented the zones of inhibition to strains S15 and S16 (14 and 12mm inhibition diameter) for Allium sativum (garlic); to strains S6 and S16 (9 and 13mm inhibition diameters) for Allium schoenoprasum (chives) and finally, strain S16 (17mm inhibition diameter) for Allium cepa (onion).Further in-depth research should be encouraged in order to make available to society effective and less expensive products which fight against antibiotic-resistant enterobacteria in the Kisangani region.
Author Keywords: Characterization, Phenotypic, Enterobacteriaceae, Antibiotic resistant, Urogenital pathways, Sensitivity, Extracts, Alicaments, Allium sativum, Allium schoenoprasum, Allium cepa, Allium porrum.
Volume 29, Issue 4, July 2020, Pages 1015–1026
O. Lokonga1 and M. Dasangba2
1 Departement of Biotechnologics Sciences, Faculty of Sciences, B.P. 2012, University of Kisangani, RD Congo
2 Département des sciences Biotechnologiques, Faculté des sciences, B.P. 2012, Université de Kisangani, RD Congo
Original language: French
Copyright © 2020 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The objective of this work is to determine the phenotypic characterization of enterobacteria with a view to identifying them, extracting aromatic oils, and preparing ethereal, chloroformic extracts of foods, in order to test the antibacterial activity of four foods, among other Allium sativum, Allium schoenoprasum, Allium cepa and Allium porrum.The aqueous, ethereal and chloroform extracts were obtained by hydro-distillation and by the extraction method using ether and chloroform respectively.As a result, the strains observed are Gram negative bacteria; their morphological and biochemical characterization made it possible to identify three germs including Citrobacter sp, Proteus mirabilis and Escherichia coli.The study of the sensitivity of these strains to hydro-distillation, to the ethereal and chloroform extract of Allium sativum, Allium schoenoprasum, Allium cepa and Allium porum by the method of diffusion in agar medium led to the following results: strains S8, S13 and S16 inhibited on hydro distillation (8; 15; 16mm of inhibition diameters); the ethereal extract of these same drugs inhibited the S14 strains for garlic (with 8mm inhibition diameter; S13 and S16 for chives (with 10mm inhibition diameter) and finally, the S16 strain for onion (with 16mm inhibition diameter), on the other hand, the chloroformic extract presented the zones of inhibition to strains S15 and S16 (14 and 12mm inhibition diameter) for Allium sativum (garlic); to strains S6 and S16 (9 and 13mm inhibition diameters) for Allium schoenoprasum (chives) and finally, strain S16 (17mm inhibition diameter) for Allium cepa (onion).Further in-depth research should be encouraged in order to make available to society effective and less expensive products which fight against antibiotic-resistant enterobacteria in the Kisangani region.
Author Keywords: Characterization, Phenotypic, Enterobacteriaceae, Antibiotic resistant, Urogenital pathways, Sensitivity, Extracts, Alicaments, Allium sativum, Allium schoenoprasum, Allium cepa, Allium porrum.
Abstract: (french)
L’objectif de ce travail est de déterminer la caractérisation phénotypique des entérobactéries en vue de les identifier, d’extraire les huiles aromatiques, et préparer des extraits éthérés, chloroformiques des alicaments, afin de tester l’activité antibactérienne de quatre alicaments entre autre Allium sativum, Allium schoenoprasum, Allium cepa et Allium porrum.Les extraits aqueux, éthérés et chloroformiques ont été obtenus par l’hydro distillation et par la méthode d’extraction à l’aide de l’éther et de chloroforme respectivement.Il ressort de résultat que les souches observées sont des bactéries Gram négatif; leur caractérisation morphologique et biochimique ont permis d’identifier trois germes dont Citrobacter sp, Proteus mirabilus et Escherichia coli.L’étude de la sensibilité des ces souches à l’hydro distillation, à l’extrait éthéré et chloroformique d’Allium sativum, Allium schoenoprasum, Allium cepa et Allium porum par la méthode de diffusion en milieu gélosé a abouti aux résultats suivants: les souches S8, S13 et S16 ont inhibé à l’hydro distillation (8; 15; 16mm de diamètres d’inhibition); l’extrait éthéré de ces mêmes alicaments ont inhibé les souches S14 pour l’ail (avec 8mm de diamètre d’inhibition; S13 et S16 pour la ciboulette (avec 10mm de diamètre d’inhibition) et enfin, la souche S16 pour l’oignon (avec 16mm de diamètre d’inhibition). Par contre, l’extrait chloroformique a présenté les zones d’inhibitions aux souches S15 et S16 (14 et 12mm de diamètre d’inhibition) pour Allium sativum (ail); aux souches S6 et S16 (9 et 13mm de diamètres d’inhibition) pour Allium schoenoprasum (ciboulette) et enfin, la souche S16 (17mm de diamètre d’inhibition) pour Allium cepa (oignon).Les recherches ultérieures approfondies sont à encourager dans le but de mettre à la disposition de la société des produits efficaces et moins coûteux qui luttent contre les entérobactéries antibiorésistantes dans la région de Kisangani.
Author Keywords: Caractérisation, Phénotypique, Entérobactéries, Antibiorésistantes, Voies Urogénitales, Sensibilité, Extraits, Alicaments, Allium sativum, Allium schoenoprasum, Allium cepa, Allium porrum.
How to Cite this Article
O. Lokonga and M. Dasangba, “Phenotypic characterization of antibiotic resistant enterobacteria isolated from the urogenital pathways and sensitivity to extracts of alicaments (Allium sativum, Allium schoenoprasum, Allium cepa and Allium porrum) at Kisangani (Tshopo Province, DR Congo),” International Journal of Innovation and Applied Studies, vol. 29, no. 4, pp. 1015–1026, July 2020.
