[ Evaluation de l'activité anti-oxydante des extraits aqueux, méthanolique et éthanolique de l'espèce saharo-endémique Myrtus nivellei Batt et Trab. (Myrtaceae) ]
Volume 6, Issue 3, July 2014, Pages 407–413
Meriem TOUAIBIA1 and Fatma Zohra CHAOUCH2
1 Département des sciences biologiques. Université SAAD DAHLEB, Algeria
2 Département des sciences agronomiques. Université SAAD DAHLEB, Algeria
Original language: French
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Myrtus nivellei Batt and Trab. is a Sahara-endemic plant belonging to the Myrtaceae family, this plant is very famous in southern Algeria for its therapeutic properties in folk medicine. However, its uses are limited exclusively to the ancestral know-how. In the present work, three extracts were prepared from the leaves of this plant: an ethanolic extract, a methanolic extract and an aqueous extract. The yields of dry crude extracts are respectively 11.12%, 12.45% and 16.5%. The quantitative estimation of flavonoids, flavonols and total phenols by the spectophotometric method showed that the three extracts contain these compounds. The evaluation of antioxidant capacity by the method of free radical scavenging test showed that all of the extracts have a very good reductive activity, especially for ethanolic extract which presented a percentage of inhibition equal to 78.81% with an EC50 estimated to 0.59 mg/ml. On the other hand, the FRAP test revealed that the methanolic extract has the best reducing power (66.71%) than those of the other extracts, but it remains relatively low compared to the ascorbic acid used as positive control.
Author Keywords: Myrtus nivellei Batt and Trab, flavonoids, flavonols, total phenols, antioxidant activity.
Volume 6, Issue 3, July 2014, Pages 407–413
Meriem TOUAIBIA1 and Fatma Zohra CHAOUCH2
1 Département des sciences biologiques. Université SAAD DAHLEB, Algeria
2 Département des sciences agronomiques. Université SAAD DAHLEB, Algeria
Original language: French
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Myrtus nivellei Batt and Trab. is a Sahara-endemic plant belonging to the Myrtaceae family, this plant is very famous in southern Algeria for its therapeutic properties in folk medicine. However, its uses are limited exclusively to the ancestral know-how. In the present work, three extracts were prepared from the leaves of this plant: an ethanolic extract, a methanolic extract and an aqueous extract. The yields of dry crude extracts are respectively 11.12%, 12.45% and 16.5%. The quantitative estimation of flavonoids, flavonols and total phenols by the spectophotometric method showed that the three extracts contain these compounds. The evaluation of antioxidant capacity by the method of free radical scavenging test showed that all of the extracts have a very good reductive activity, especially for ethanolic extract which presented a percentage of inhibition equal to 78.81% with an EC50 estimated to 0.59 mg/ml. On the other hand, the FRAP test revealed that the methanolic extract has the best reducing power (66.71%) than those of the other extracts, but it remains relatively low compared to the ascorbic acid used as positive control.
Author Keywords: Myrtus nivellei Batt and Trab, flavonoids, flavonols, total phenols, antioxidant activity.
Abstract: (french)
Myrtus nivellei Batt et Trab. est une plante saharo-endémique, appartenant à la famille des Myrtaceae, Cette plante est très réputée au sud algérien pour ses vertus thérapeutiques en médecine populaire. Cependant, ses usages restent toutefois, exclusivement limités au savoir-faire ancestral. Dans le présent travail trois extraits ont été préparés, à partir des feuilles de cette plante: un extrait éthanolique, un extrait méthanolique et un extrait aqueux. Les rendements en extraits brutes secs sont de l'ordre de 11,12%, 12,45% et 16,5% respectivement. L'estimation quantitative des flavonoïdes, des flavonols et des phénols totaux par la méthode spectrophotométrique a montré que ces trois extraits contiennent ces composés. L'évaluation du pouvoir anti-oxydant par la méthode du piégeage des radicaux libres a montré que les extraits étudiés ont tous une très bonne activité réductrice, surtout pour l'extrait éthanolique ayant présenté un pourcentage d'inhibition égale à 78,81% avec une EC50 égale à 0,59 mg/ml. D'autre part, le test de FRAP a révélé que l'extrait méthanolique a le meilleur pouvoir réducteur (66,71%) par rapport ceux des autres extraits, mais qui reste, toutefois, relativement faible par rapport à celui de l'acide ascorbique utilisé comme contrôle positif.
Author Keywords: Myrtus nivellei Batt et Trab., flavonoïdes, flavonols, phénols totaux, activité anti-oxydante.
How to Cite this Article
Meriem TOUAIBIA and Fatma Zohra CHAOUCH, “Evaluation of the antioxidant activity of aqueous, methanolic and ethanolic extracts of the Sahara-endemic species Myrtus nivellei Batt and Trab. (Myrtaceae),” International Journal of Innovation and Applied Studies, vol. 6, no. 3, pp. 407–413, July 2014.
