[ Mise au point et validation d'une méthode de dosage du Méthanol dans le sang par Chromatographie en Phase Gazeuse avec injecteur « Headspace » couplée à un Détecteur à Ionisation de Flamme ]
Volume 7, Issue 3, August 2014, Pages 1038–1044
Narjis BADRANE1, Fatima Zahra AMRI2, Mostafa Khaya3, Mohammed Ghandi4, Seloua Elmrabeh5, Naima Ait Daoud6, Mouncef Idrissi7, Fouad OUAZZANI CHAHDI8, Youssef KANDRI RODI9, and Rachida SOULAYMANI BENCHEIKH10
1 Laboratoire de Toxicologie et de Pharmacologie, Centre Anti-Poison et de Pharmacovigilance du Maroc, Rabat, Morocco
2 Faculté des Sciences et Techniques, Fès, Morocco
3 Faculty of science, Mohammed V University – Agdal, B.P. 1014, Rabat, Morocco
4 Poison Control and Pharmacovigilance Centre of Morocco, B.P. 6671, Rabat, Morocco
5 Poison Control and Pharmacovigilance Centre of Morocco, B.P. 6671, Rabat, Morocco
6 Poison Control and Pharmacovigilance Centre of Morocco, B.P. 6671, Rabat, Morocco
7 Poison Control and Pharmacovigilance Centre of Morocco, B.P. 6671, Rabat, Morocco
8 Laboratoire de Chimie Organique appliquée, Université Sidi Mohamed Ben Abdellah, Faculté des Sciences et Techniques, Fès, Morocco
9 Faculté des Sciences et Techniques, Fès, Morocco
10 Laboratoire de Toxicologie et de Pharmacologie, Centre Anti-Poison et de Pharmacovigilance du Maroc, Rabat, Morocco
Original language: French
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Introduction: Methanol is a commonly used organic solvent that, because of its toxicity, can cause metabolic acidosis, neurologic sequelae, and even death, when ingested. It is a constituent of many commercially available industrial solvents and of poorly adulterated alcoholic beverages. The management of acute human methanol intoxication needs methanol dosage. The aim of our study is to validate a method in the order to determinate the amount of blood methanol, using gas chromatography with flame ionization detection (FID), after a Headspace sampling.
Methods: We used butanol as an internal standard. The validation procedure was performed according to the guidelines of the French Society of Analytical Toxicology and the French Committee of Accreditation (COFRAC; LABGTA04).
Results: Our method is specific and linear at the range amount from 0g/L to 1 g/L. the coefficient of correlation was 0.99868. The intra- and inter-day precision and relative bias were less than 15 %. The limit of detection was 0.060 g/L. In the same conditions, limit of quantification was 0.09 g/L. The storage's and transport's samples were at +4
Author Keywords: toxicity, methanol, dosage, validation, chromatography, headspace.
Volume 7, Issue 3, August 2014, Pages 1038–1044
Narjis BADRANE1, Fatima Zahra AMRI2, Mostafa Khaya3, Mohammed Ghandi4, Seloua Elmrabeh5, Naima Ait Daoud6, Mouncef Idrissi7, Fouad OUAZZANI CHAHDI8, Youssef KANDRI RODI9, and Rachida SOULAYMANI BENCHEIKH10
1 Laboratoire de Toxicologie et de Pharmacologie, Centre Anti-Poison et de Pharmacovigilance du Maroc, Rabat, Morocco
2 Faculté des Sciences et Techniques, Fès, Morocco
3 Faculty of science, Mohammed V University – Agdal, B.P. 1014, Rabat, Morocco
4 Poison Control and Pharmacovigilance Centre of Morocco, B.P. 6671, Rabat, Morocco
5 Poison Control and Pharmacovigilance Centre of Morocco, B.P. 6671, Rabat, Morocco
6 Poison Control and Pharmacovigilance Centre of Morocco, B.P. 6671, Rabat, Morocco
7 Poison Control and Pharmacovigilance Centre of Morocco, B.P. 6671, Rabat, Morocco
8 Laboratoire de Chimie Organique appliquée, Université Sidi Mohamed Ben Abdellah, Faculté des Sciences et Techniques, Fès, Morocco
9 Faculté des Sciences et Techniques, Fès, Morocco
10 Laboratoire de Toxicologie et de Pharmacologie, Centre Anti-Poison et de Pharmacovigilance du Maroc, Rabat, Morocco
Original language: French
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Introduction: Methanol is a commonly used organic solvent that, because of its toxicity, can cause metabolic acidosis, neurologic sequelae, and even death, when ingested. It is a constituent of many commercially available industrial solvents and of poorly adulterated alcoholic beverages. The management of acute human methanol intoxication needs methanol dosage. The aim of our study is to validate a method in the order to determinate the amount of blood methanol, using gas chromatography with flame ionization detection (FID), after a Headspace sampling.
Methods: We used butanol as an internal standard. The validation procedure was performed according to the guidelines of the French Society of Analytical Toxicology and the French Committee of Accreditation (COFRAC; LABGTA04).
Results: Our method is specific and linear at the range amount from 0g/L to 1 g/L. the coefficient of correlation was 0.99868. The intra- and inter-day precision and relative bias were less than 15 %. The limit of detection was 0.060 g/L. In the same conditions, limit of quantification was 0.09 g/L. The storage's and transport's samples were at +4
Author Keywords: toxicity, methanol, dosage, validation, chromatography, headspace.
How to Cite this Article
Narjis BADRANE, Fatima Zahra AMRI, Mostafa Khaya, Mohammed Ghandi, Seloua Elmrabeh, Naima Ait Daoud, Mouncef Idrissi, Fouad OUAZZANI CHAHDI, Youssef KANDRI RODI, and Rachida SOULAYMANI BENCHEIKH, “Validation of a quantitative determination method of methanol in blood using Gas Chromatography with Flame Ionization detection after a Headspace sampling,” International Journal of Innovation and Applied Studies, vol. 7, no. 3, pp. 1038–1044, August 2014.
