Volume 13, Issue 2, October 2015, Pages 348–354
Khadiga Gaafar Abd Elaleem1, Badr Eldin A.E. Saeed2, and Magda Mohamed Ahmed3
1 Department of Biology and Biotechnology, Faculty of Science and Technology, AL Neelain University, Khartoum, SUDAN
2 Department of Biology and Biotechnology, Faculty of Science and Technology, AL Neelain University, Khartoum, SUDAN
3 Commission of Biotechnology and Genetic Engineering, National Centre for Research, Khartoum, SUDAN
Original language: English
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The present study aimed to investigate the effect of different plant growth regulator namely 2,4 dichlorophenoxy acetic acid (2,4-D) and ?-Naphthalene Acetic Acid (NAA) on callus induction of Helianthus annuus L.
Callus culture were initiated from three explants namely cotyledon leaf, hypocotyl and root segments explants of in vitro Helianthus annuus L seedling.
Both auxins 2,4-D and NAA stimulate callus induction, however 1.5 mg/L 2,4-D proved to be more effected for induction of callus. Among all treatment media containing 1.5 mg/l 2,4 D showed highest response of callus degree (3.44
Author Keywords: 4 Dichlorophenoxy acetic acid (2, 4-D), Root segment, explant, Hypocotyls, Helianthus annuus L, Callus induction.
Khadiga Gaafar Abd Elaleem1, Badr Eldin A.E. Saeed2, and Magda Mohamed Ahmed3
1 Department of Biology and Biotechnology, Faculty of Science and Technology, AL Neelain University, Khartoum, SUDAN
2 Department of Biology and Biotechnology, Faculty of Science and Technology, AL Neelain University, Khartoum, SUDAN
3 Commission of Biotechnology and Genetic Engineering, National Centre for Research, Khartoum, SUDAN
Original language: English
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The present study aimed to investigate the effect of different plant growth regulator namely 2,4 dichlorophenoxy acetic acid (2,4-D) and ?-Naphthalene Acetic Acid (NAA) on callus induction of Helianthus annuus L.
Callus culture were initiated from three explants namely cotyledon leaf, hypocotyl and root segments explants of in vitro Helianthus annuus L seedling.
Both auxins 2,4-D and NAA stimulate callus induction, however 1.5 mg/L 2,4-D proved to be more effected for induction of callus. Among all treatment media containing 1.5 mg/l 2,4 D showed highest response of callus degree (3.44
Author Keywords: 4 Dichlorophenoxy acetic acid (2, 4-D), Root segment, explant, Hypocotyls, Helianthus annuus L, Callus induction.
How to Cite this Article
Khadiga Gaafar Abd Elaleem, Badr Eldin A.E. Saeed, and Magda Mohamed Ahmed, “Effect of plant growth regulators on Helianthus annuus L. callus induction,” International Journal of Innovation and Applied Studies, vol. 13, no. 2, pp. 348–354, October 2015.
