[ Analyse moléculaire de deux génotypes de Medicago truncatula Gaertn. par le marqueur de séquence exprimée EST-SSR (MTIC 124) en réponse à la salinité ]
Volume 17, Issue 2, July 2016, Pages 627–631
Adel Amar AMOURI1 and Seghir HADJADJ AOUL2
1 Laboratoire d’écologie végétale, Département de Biologie, Faculté des Sciences de la nature et de la vie, BP1524, Université d’Oran 1 Ahmed Ben Bella, Oran, Algeria
2 Laboratoire d’écologie végétale, Département de Biologie, Faculté des Sciences de la nature et de la vie, BP1524, Université d’Oran 1 Ahmed Ben Bella, Oran, Algeria
Original language: French
Copyright © 2016 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In this study, we used the expressed sequence EST-SSR marker (MTIC124) to show genetic variation and determine a potential link between this marker and salt stress tolerance on two contrasting Medicago truncatula Gaertn. genotypes (Tru 131 tolerant genotype, and Jemalong, sensitive one). The amplification of the DNA isolated from 10 individual seedlings for each genotype with the EST- SSR primers (MTIC 124) produced a total of 20 amplified products. The sizes of the alleles detected ranged from 100 to 280 bp. The analysis of polymorphism locus showed that the tolerant genotype (Tru 131) population had two alleles, genetic diversity index of 0.32 and PIC value of 0.267. The results obtained from UniGene and Uniprot databases of highly similarity proteins sequences with the EST- SSR locus MTIC 124, showed that this locus encode cysteine proteinase inhibitor and was expressed principally in root of M.truncatula. This data suggest that this locus is involved in salinity tolerance and it is appropriate for studying and understanding salt stress tolerance mechanisms in Medicago truncatula Gaertn.
Author Keywords: Medicago truncatula Gaertn., salt stress, molecular marker, molecular databases.
Volume 17, Issue 2, July 2016, Pages 627–631
Adel Amar AMOURI1 and Seghir HADJADJ AOUL2
1 Laboratoire d’écologie végétale, Département de Biologie, Faculté des Sciences de la nature et de la vie, BP1524, Université d’Oran 1 Ahmed Ben Bella, Oran, Algeria
2 Laboratoire d’écologie végétale, Département de Biologie, Faculté des Sciences de la nature et de la vie, BP1524, Université d’Oran 1 Ahmed Ben Bella, Oran, Algeria
Original language: French
Copyright © 2016 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
In this study, we used the expressed sequence EST-SSR marker (MTIC124) to show genetic variation and determine a potential link between this marker and salt stress tolerance on two contrasting Medicago truncatula Gaertn. genotypes (Tru 131 tolerant genotype, and Jemalong, sensitive one). The amplification of the DNA isolated from 10 individual seedlings for each genotype with the EST- SSR primers (MTIC 124) produced a total of 20 amplified products. The sizes of the alleles detected ranged from 100 to 280 bp. The analysis of polymorphism locus showed that the tolerant genotype (Tru 131) population had two alleles, genetic diversity index of 0.32 and PIC value of 0.267. The results obtained from UniGene and Uniprot databases of highly similarity proteins sequences with the EST- SSR locus MTIC 124, showed that this locus encode cysteine proteinase inhibitor and was expressed principally in root of M.truncatula. This data suggest that this locus is involved in salinity tolerance and it is appropriate for studying and understanding salt stress tolerance mechanisms in Medicago truncatula Gaertn.
Author Keywords: Medicago truncatula Gaertn., salt stress, molecular marker, molecular databases.
Abstract: (french)
Dans cette étude, nous avons utilisé le marqueur de séquence exprimée EST-SSR (MTIC 124) pour évaluer la variabilité génétique et déterminer la relation potentielle entre ce marqueur et la tolérance au stress salin, de deux génotypes contrastés de Medicago truncatula Gaertn. (Tru 131, tolérant & Jemalong, sensible). L’amplification de l’ADN isolé à partir de 10 individus de jeunes plants de chaque génotype avec les amorces d’EST-SSR (MTIC124) a produit un total de 20 amplicons. La taille des allèles détectée, varie de 100 à 280 pb. L’analyse du polymorphisme de ce locus a montré que les population du génotype Tru 131 a deux allèles, une diversité génétique de 0.32 et un PIC de 0.267. Les résultats obtenus à partir des bases de données Unigene et Uniprot sur les séquence protéiques hautement similaires avec le locus EST-SSR (MTIC 124), ont montré que ce locus code pour l’inhibiteur de la cystéine protéinase, qui est exprimée principalement au niveau des racines. Cette information suggère que ce locus est impliqué dans l’adaptation à la salinité, et il est adéquat pour l’étude et la compréhension des mécanismes de tolérance au stress salin chez Medicago truncatula Gaertn.
Author Keywords: Medicago truncatula Gaertn., stress salin, marqueur moléculaire, bases de données moléculaires.
How to Cite this Article
Adel Amar AMOURI and Seghir HADJADJ AOUL, “Molecular analysis of two genotypes of Medicago truncatula Gaertn. by the expressed sequence tag EST-SSR (MTIC 124) in response to salinity,” International Journal of Innovation and Applied Studies, vol. 17, no. 2, pp. 627–631, July 2016.
