[ Evaluation in vivo de l’activité antiplasmodiale des extraits de Artemisia annua L., Alisicarpus ovalifolius (SCHUM. et THONN.) J. LEONARD. et de Securidaka longepediculata FRESS. chez la souris nmri infestée par le Plasmodium berghei NK 65 ]
Volume 39, Issue 2, April 2023, Pages 800–810
Amadou Mounkaila Hamissou1, Souley Kallo Moutari2, Oubayyou Abdoulaye Mamoudou3, Idrissa Moussa4, Mahamane Ali5, and Ikhri Kalid6
1 Department de Productions Végétales, Faculté d’Agronomie, Université Abdou Moumouni, BP: 10960 Niamey, Niger
2 Faculté de Sciences et Techniques, Université Abdou Moumouni, Niamey, BP 10662, Niger
3 Laboratoire de Biochimie, Hôpital National Amirou Boubacar Diallo, BP 10146, Niamey, Niger
4 Department de Chimie, Faculté des Sciences et Techniques, Université Abdou Moumouni, BP 10662, Niamey, Niger
5 Université de Diffa, Faculté des Sciences Agronomiques, BP 78, Diffa, Niger
6 Laboratoire de Biochimie, Hôpital National Amirou Boubacar Diallo, BP 10146, Niamey, Niger
Original language: French
Copyright © 2023 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
This work aims to evaluate the antimalarial biological activities of medicinal plant extracts namely Artemisia annua, Alisicarpus ovalifolius and Securidaka longepediculata. The phytochemical analysis of the extracts of these plants revealed the presence of saponins, alkaloids, flavonoids, quinones, phenols and terpenes. Toxicity tests according to the method proposed by OECD have made it possible to conclude that the plant extracts are not toxic. The tests were carried out on mice, inoculated intraperitoneally with Plasmodium berghei. These animals were divided into eleven groups of five, nine of which each received a dose of the extract to be tested and the other two, distilled water for the negative control and arthemether for the positive control. The different extracts showed various antimalarial activities using the four-day Peters test on infected mice. Artemisia annua gave the highest parasitaemia reduction percentages with 87.2; 96.8 and 96% respectively at doses of 100, 250 and 500 mg/kg, while Alisicarpus ovalifolius recorded 61.6; 71.20 and 80.8% respectively at the same doses. Securidaka longepediculata extracts gave low percentage reductions in parasitaemia. Statistical analysis reveals that the extracts showed a significant difference compared to the negative control (P<0.05).
Author Keywords: Artemether, healing plants, antimalarial, Plasmodium berghei, test in vivo.
Volume 39, Issue 2, April 2023, Pages 800–810
Amadou Mounkaila Hamissou1, Souley Kallo Moutari2, Oubayyou Abdoulaye Mamoudou3, Idrissa Moussa4, Mahamane Ali5, and Ikhri Kalid6
1 Department de Productions Végétales, Faculté d’Agronomie, Université Abdou Moumouni, BP: 10960 Niamey, Niger
2 Faculté de Sciences et Techniques, Université Abdou Moumouni, Niamey, BP 10662, Niger
3 Laboratoire de Biochimie, Hôpital National Amirou Boubacar Diallo, BP 10146, Niamey, Niger
4 Department de Chimie, Faculté des Sciences et Techniques, Université Abdou Moumouni, BP 10662, Niamey, Niger
5 Université de Diffa, Faculté des Sciences Agronomiques, BP 78, Diffa, Niger
6 Laboratoire de Biochimie, Hôpital National Amirou Boubacar Diallo, BP 10146, Niamey, Niger
Original language: French
Copyright © 2023 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
This work aims to evaluate the antimalarial biological activities of medicinal plant extracts namely Artemisia annua, Alisicarpus ovalifolius and Securidaka longepediculata. The phytochemical analysis of the extracts of these plants revealed the presence of saponins, alkaloids, flavonoids, quinones, phenols and terpenes. Toxicity tests according to the method proposed by OECD have made it possible to conclude that the plant extracts are not toxic. The tests were carried out on mice, inoculated intraperitoneally with Plasmodium berghei. These animals were divided into eleven groups of five, nine of which each received a dose of the extract to be tested and the other two, distilled water for the negative control and arthemether for the positive control. The different extracts showed various antimalarial activities using the four-day Peters test on infected mice. Artemisia annua gave the highest parasitaemia reduction percentages with 87.2; 96.8 and 96% respectively at doses of 100, 250 and 500 mg/kg, while Alisicarpus ovalifolius recorded 61.6; 71.20 and 80.8% respectively at the same doses. Securidaka longepediculata extracts gave low percentage reductions in parasitaemia. Statistical analysis reveals that the extracts showed a significant difference compared to the negative control (P<0.05).
Author Keywords: Artemether, healing plants, antimalarial, Plasmodium berghei, test in vivo.
Abstract: (french)
Ce travail a pour but d’évaluer les activités biologiques antipaludiques des extraits de plantes médicinales à savoir Artemisia annua, Alisicarpus ovalifolius et de Securidaka longepediculata. L’analyse phytochimique des extraits de ces plantes, a révélé la présence de saponines, d’alcaloïdes, de flavonoïdes, de quinones, de phénols et de terpènes. Les tests de toxicité selon la méthode proposée par OCDE ont permis de conclure que les extraits des plantes ne sont pas toxiques. Les tests ont été effectués sur des souris, inoculées par voie intrapéritonéale avec le Plasmodium berghei. Ces animaux ont été repartis en onze groupes de cinq, dont neuf ont reçu chacun une dose de l’extrait à tester et les deux autres, de l’eau distillée pour le témoin négatif et de l’arthémether pour le témoin positif. Les différents extraits ont montré diverses activités antipaludiques en utilisant le test de Peters de quatre jours sur les souris infectées. Artemisia annua a donné les pourcentages de réduction de la parasitémie, les plus élevés avec 87,2; 96,8 et 96 % respectivement aux doses de 100, 250 et 500 mg/kg, tandis que Alisicarpus ovalifolius a enregistrée 61,6; 71,20 et 80,8 % respectivement aux mêmes doses. Les extraits de Securidaka longepediculata ont donné de faibles pourcentages de réduction de la parasitémie. L’analyse statistique révèle que les extraits ont montré une différence significative comparés au témoin négatif (P<0.05).
Author Keywords: Arthémether, plantes médicinales, antipaludique, Plasmodium berghei, test in vivo.
How to Cite this Article
Amadou Mounkaila Hamissou, Souley Kallo Moutari, Oubayyou Abdoulaye Mamoudou, Idrissa Moussa, Mahamane Ali, and Ikhri Kalid, “Evaluation in vivo of the antiplasmodial activity of extracts of Artemisia annua L., Alisicarpus ovalifolius (SCHUM. et THONN.) J. LEONARD. and Securidaka longepediculata FRESS. in nmri mice infected with Plasmodium berghei NK 65,” International Journal of Innovation and Applied Studies, vol. 39, no. 2, pp. 800–810, April 2023.
