Volume 44, Issue 1, November 2024, Pages 33–42
Tchimonbié Messikély ANOMAN1, Aya Lucie Félicité N’GAZA2, Any Olivier KOMENAN3, Yao Anicet Gervais KOUAME4, Konan Samuel AHOUDJO5, and Doffou Selastique AKAFFOU6
1 Unité de Formation et de Recherche (UFR) Agroforesterie de l’Université Jean Lorougnon Guédé de Daloa, Département de Biologie, Génétique et Physiologie, Génétique et Amélioration des Espèces, BP 150 Daloa, Côte d’Ivoire
2 National Center for Agricultural Research (CNRA), Vegetable and Protein, Research Program, Food Crops Research Station, 01 BP 633 Bouaké 01, Côte d’Ivoire
3 Unité de Formation et de Recherche (UFR) Agroforesterie de l’Université Jean Lorougnon Guédé de Daloa, Département de Biologie, Génétique et Physiologie, Génétique et Amélioration des Espèces, BP 150 Daloa, Côte d’Ivoire
4 Unité de Formation et de Recherche (UFR) Agroforesterie de l’Université Jean Lorougnon Guédé de Daloa, Département de Biologie Végétale, Laboratoire de l’Amélioration de la Production Agricole, BP 150 Daloa, Côte d’Ivoire
5 UMR Marchés, Organisations, Institutions et Stratégies d’Acteurs (MOISA), Montpellier SupAgro, Campus de La Gaillarde, 34060 Montpellier Cedex 02, France
6 Unité de Formation et de Recherche (UFR) Agroforesterie de l’Université Jean Lorougnon Guédé de Daloa, Département de Biologie, Génétique et Physiologie, Génétique et Amélioration des Espèces, BP 150 Daloa, Côte d’Ivoire
Original language: English
Copyright © 2024 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The watershed of the central lake system of the city of Yamoussoukro in Côte d’Ivoire is subject to various forms of pollution. Ralstonia solanacearum (R. solanacearum), responsible for bacterial wilt of tomato (Solanum lycopersicum; 2n=2X=24Chr.), persists in market garden environments and causes tomato yield losses of up to 100%. The aim of this study was to assess the genetic diversity of R. solanacearum, its impact on tomato cultivation in Yamoussoukro, and to obtain genotypes of Solanum lycopersicum resistant to R. solanacearum. Samples of water, soil and wilted tomato plants was collected from June 2022 to August 2023, at six growing sites around three selected lakes. R. solanacearum was isolated on SMSA medium and confirmed by PCR. Genetic diversity of strains was assessed by rep-PCR. Biovar, race and pathogenicity tests were used to assess the virulence levels of strains at each site studied, and to select tomato plants potentially resistant to R. solanacearum. The results showed a significant variation in the incidence and severity of bacterial wilt from one site to another. The 63 R. solanacearum strains isolated and confirmed by PCR belonged mainly to biovar 3 and race 1. Phylogenetic analysis of R. solanacearum showed three distinct groups with around 70% similarity. Two groups was made up solely of strains from Lake E. A single group included all strains from all lakes. Strains from the LacE-Ch2 site showed a particularly high aggressiveness of 88%. Of the 42 tomato cultivars tested in the greenhouse, three showed no symptoms of bacterial wilt.
Author Keywords: bacterial wilt, genetic resistance, pathogenicity, rep-PCR, taxonomy.
Tchimonbié Messikély ANOMAN1, Aya Lucie Félicité N’GAZA2, Any Olivier KOMENAN3, Yao Anicet Gervais KOUAME4, Konan Samuel AHOUDJO5, and Doffou Selastique AKAFFOU6
1 Unité de Formation et de Recherche (UFR) Agroforesterie de l’Université Jean Lorougnon Guédé de Daloa, Département de Biologie, Génétique et Physiologie, Génétique et Amélioration des Espèces, BP 150 Daloa, Côte d’Ivoire
2 National Center for Agricultural Research (CNRA), Vegetable and Protein, Research Program, Food Crops Research Station, 01 BP 633 Bouaké 01, Côte d’Ivoire
3 Unité de Formation et de Recherche (UFR) Agroforesterie de l’Université Jean Lorougnon Guédé de Daloa, Département de Biologie, Génétique et Physiologie, Génétique et Amélioration des Espèces, BP 150 Daloa, Côte d’Ivoire
4 Unité de Formation et de Recherche (UFR) Agroforesterie de l’Université Jean Lorougnon Guédé de Daloa, Département de Biologie Végétale, Laboratoire de l’Amélioration de la Production Agricole, BP 150 Daloa, Côte d’Ivoire
5 UMR Marchés, Organisations, Institutions et Stratégies d’Acteurs (MOISA), Montpellier SupAgro, Campus de La Gaillarde, 34060 Montpellier Cedex 02, France
6 Unité de Formation et de Recherche (UFR) Agroforesterie de l’Université Jean Lorougnon Guédé de Daloa, Département de Biologie, Génétique et Physiologie, Génétique et Amélioration des Espèces, BP 150 Daloa, Côte d’Ivoire
Original language: English
Copyright © 2024 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The watershed of the central lake system of the city of Yamoussoukro in Côte d’Ivoire is subject to various forms of pollution. Ralstonia solanacearum (R. solanacearum), responsible for bacterial wilt of tomato (Solanum lycopersicum; 2n=2X=24Chr.), persists in market garden environments and causes tomato yield losses of up to 100%. The aim of this study was to assess the genetic diversity of R. solanacearum, its impact on tomato cultivation in Yamoussoukro, and to obtain genotypes of Solanum lycopersicum resistant to R. solanacearum. Samples of water, soil and wilted tomato plants was collected from June 2022 to August 2023, at six growing sites around three selected lakes. R. solanacearum was isolated on SMSA medium and confirmed by PCR. Genetic diversity of strains was assessed by rep-PCR. Biovar, race and pathogenicity tests were used to assess the virulence levels of strains at each site studied, and to select tomato plants potentially resistant to R. solanacearum. The results showed a significant variation in the incidence and severity of bacterial wilt from one site to another. The 63 R. solanacearum strains isolated and confirmed by PCR belonged mainly to biovar 3 and race 1. Phylogenetic analysis of R. solanacearum showed three distinct groups with around 70% similarity. Two groups was made up solely of strains from Lake E. A single group included all strains from all lakes. Strains from the LacE-Ch2 site showed a particularly high aggressiveness of 88%. Of the 42 tomato cultivars tested in the greenhouse, three showed no symptoms of bacterial wilt.
Author Keywords: bacterial wilt, genetic resistance, pathogenicity, rep-PCR, taxonomy.
How to Cite this Article
Tchimonbié Messikély ANOMAN, Aya Lucie Félicité N’GAZA, Any Olivier KOMENAN, Yao Anicet Gervais KOUAME, Konan Samuel AHOUDJO, and Doffou Selastique AKAFFOU, “Influence of pollution of the Yamoussoukro lakes watershed on the genetic diversity of ralstonia solanacearum: Implications for tomato productivity in Yamoussoukro,” International Journal of Innovation and Applied Studies, vol. 44, no. 1, pp. 33–42, November 2024.
