[ Activité antifongique de la poudre de feuille de Neem (Azadirachta indica) sur les contaminants fongiques isolés des graines de Niebe (Vigna unguiculata) pendant le stockage après la récolte ]
Volume 44, Issue 3, January 2025, Pages 629–642
Touré Naka1, Kokora Aya Philomène2, Kouamé Liliane Maimouna3, Djédjé Blètro Patrick Gérad4, and Atobla Koua5
1 Laboratory of Biotechnology and Valuation of Agri-Resources and Natural Substances, Department of Biological Sciences of Pelefero Gon Coulibaly University, Korhogo, Côte d’Ivoire
2 Laboratory of Biotechnology and Valuation of Agri-Resources and Natural Substances, Department of Biological Sciences of Pelefero Gon Coulibaly University, Korhogo, Côte d’Ivoire
3 Laboratory of Biochemistry, Microbiology and Valorization of Agricultural Resources, Institute of Agropastoral Management, Pelefero Gon Coulibaly University, Korhogo, Côte d’Ivoire
4 Laboratory of Biocatalysis and Bioprocesses, Department of Food Science and Technology, Nangui Abrogoua University, 02 BP 801 Abidjan 02, Côte d’Ivoire
5 Laboratory of Biotechnology, Agriculture and Valuation of Biological Resources, Department of Biosciences, Felix Houphouet-Boigny University, Abidjan, Côte d´Ivoire
Original language: French
Copyright © 2025 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In sub-Saharan Africa in general and in Côte d’Ivoire in particular, post-harvest losses of agricultural products are still a major problem. It is with this in mind that this study was conducted to identify the fungal flora of cowpea seeds intended for human consumption and to evaluate the activity of neem leaf powder on this flora. Thus, four batches of cowpea were purchased in the three main markets of Korhogo. At T = 0 week, nine Petri dishes with Dichloran Rose Bengal Chloramphenicol were inoculated with the batch of untreated cowpea seeds. Each Petri dish was then incubated at 30 ° C for five days. We also proceeded in the same way, with the seeds treated at a rate of one culture per week for 21 days. At T = 5 days, three fungi were isolated and identified at varying percentages of occurrence: Aspergillus flavus (34.79%), Aspergillus niger (32.95%) and Rhizopus solinifer (6.79%). The evaluation of neem leaf powder showed its ability to inhibit the fungal growth of pathogens isolated from cowpea seeds. The leaf powder therefore has properties that can be used for the conservation of cowpea seeds.
Author Keywords: Cowpea, fungal, neem, biopesticides, post-harvest losses.
Volume 44, Issue 3, January 2025, Pages 629–642
Touré Naka1, Kokora Aya Philomène2, Kouamé Liliane Maimouna3, Djédjé Blètro Patrick Gérad4, and Atobla Koua5
1 Laboratory of Biotechnology and Valuation of Agri-Resources and Natural Substances, Department of Biological Sciences of Pelefero Gon Coulibaly University, Korhogo, Côte d’Ivoire
2 Laboratory of Biotechnology and Valuation of Agri-Resources and Natural Substances, Department of Biological Sciences of Pelefero Gon Coulibaly University, Korhogo, Côte d’Ivoire
3 Laboratory of Biochemistry, Microbiology and Valorization of Agricultural Resources, Institute of Agropastoral Management, Pelefero Gon Coulibaly University, Korhogo, Côte d’Ivoire
4 Laboratory of Biocatalysis and Bioprocesses, Department of Food Science and Technology, Nangui Abrogoua University, 02 BP 801 Abidjan 02, Côte d’Ivoire
5 Laboratory of Biotechnology, Agriculture and Valuation of Biological Resources, Department of Biosciences, Felix Houphouet-Boigny University, Abidjan, Côte d´Ivoire
Original language: French
Copyright © 2025 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
In sub-Saharan Africa in general and in Côte d’Ivoire in particular, post-harvest losses of agricultural products are still a major problem. It is with this in mind that this study was conducted to identify the fungal flora of cowpea seeds intended for human consumption and to evaluate the activity of neem leaf powder on this flora. Thus, four batches of cowpea were purchased in the three main markets of Korhogo. At T = 0 week, nine Petri dishes with Dichloran Rose Bengal Chloramphenicol were inoculated with the batch of untreated cowpea seeds. Each Petri dish was then incubated at 30 ° C for five days. We also proceeded in the same way, with the seeds treated at a rate of one culture per week for 21 days. At T = 5 days, three fungi were isolated and identified at varying percentages of occurrence: Aspergillus flavus (34.79%), Aspergillus niger (32.95%) and Rhizopus solinifer (6.79%). The evaluation of neem leaf powder showed its ability to inhibit the fungal growth of pathogens isolated from cowpea seeds. The leaf powder therefore has properties that can be used for the conservation of cowpea seeds.
Author Keywords: Cowpea, fungal, neem, biopesticides, post-harvest losses.
Abstract: (french)
: En Afrique subsaharienne en général et en Côte d’Ivoire en particulier, les pertes post-récolte des produits agricoles constituent toujours un problème majeur. C’est dans cette optique que cette étude a été menée pour identifier la flore fongique des graines de niébé destinées à la consommation humaine et évaluer l’activité de la poudre de feuilles de neem sur cette flore. Ainsi, quatre lots de niébé ont été achetés dans les trois principaux marchés de Korhogo. A T=0 semaine, neuf boîtes de Petri au Dichloran Rose Bengal Chloramphénicol ont été inoculées avec le lot de graines de niébé non traitées. Chaque boîte de Petri a ensuite été incubée à 30°C pendant cinq jours. Nous avons également procédé de la même manière, avec les graines traitées à raison d’une culture par semaine pendant 21 jours. A T=5 jours, trois champignons ont été isolés et identifiés à des pourcentages d’occurrence variables: Aspergillus flavus (34,79%), Aspergillus niger (32,95%) et Rhizopus solinifer (6,79%). L’évaluation de la poudre de feuilles de neem a montré sa capacité à inhiber la croissance fongique des agents pathogènes isolés des graines de niébé. La poudre de feuilles possède donc des propriétés qui peuvent être utilisées pour la conservation des graines de niébé.
Author Keywords: Niébé, champignon, neem, biopesticides, pertes après récolte.
How to Cite this Article
Touré Naka, Kokora Aya Philomène, Kouamé Liliane Maimouna, Djédjé Blètro Patrick Gérad, and Atobla Koua, “Antifungal activity of Neem (Azadirachta indica) leaf powder on fungal contaminants isolated from Cowpea (Vigna unguiculata) seeds during postharvest storage,” International Journal of Innovation and Applied Studies, vol. 44, no. 3, pp. 629–642, January 2025.
