This study was conducted to show whether hormonal replacement treatment would offer ameliorating effects against abnormal alterations associated with osteoporosis in female albino rats. Two experiments were carried out, in the first experiment, the rats were randomly divided into two main groups. The first group, were let without surgery and served as control while, the second group were obeyed to bilateral ovariectomy surgery to induce osteoporosis; after 4 weeks induced acute hormonal disturbance indicated by decreased estradiols and progesterone levels. While, the level of follicular stimulating hormone and luteinizing hormone were increase in osteoporosis female rats regarding to levels in normal control female rats. In the second experiment, a remarkable correction was occurred in the levels of follicular stimulating hormone and luteinizing hormone by gradually decreased after 90 days of treatment. While, a considerable amelioration effect was occurred in the estradiols level by gradually increasing of levels with the time and treatment with estrogen replacement. These corrections were dependent on the time of treatment (30, 60 & 90 days). According to the pre-mentioned results, it is importance to concluding that the treatment with estradiol replacement ameliorates chance of recovery from hazardous effects of osteoporoses. The obtained data were discussed according to available obtained researches.