The study was carried out as part of the sustainable increase in plantain production and as part of the strategy to contribute to achieving food security. In banana growing, the use of successive suckers for the next growing cycles without any measure of sanitation of the planting material contributes to the increase of nematodes in plantation. this study objective is to manage nematode populations through cultural practices. The CORNE 1 variety was planted at high density (2,500 plants / ha) in a device involving four treatments: two cycles without endomycorrhizae application, two cycles with endomycorrhizae application, annual replanting with endomycorrhizae application, annual replanting without endomycorrhizae application. Replanting took place 0.8 m from the fruiting stand of the previous growing season. The trial lasted three growing seasons. Nematode infestations are concentrated (80 %) within a radius of 50 cm around the fruiting foot. In addition, the lowest infestations of nematodes were observed with annual replanting with or without endomycorrhizae. This resulted in yields which remained high (P<0.05; Fisher test) in the second (35 t/ha) and third growing season (33 t/ha) and which were statistically comparable to that obtained at the first season (35 t/ha). In continuous cultivation, on the contrary, reductions in yields of 41.4 % and 63.5 % were recorded respectively in the second and third growing seasons. The use of vivoplants in annual replanting with endomychores offers the possibility of cultivating organic plantain without using nematicides.