Introduction: Smoking constitutes a serious risk to the health of workers. The objective of this study was to evaluate the knowledge of caregivers on the harmful effects of tobacco and their perception of the actions to be promoted for a tobacco-free hospital. Methodology: This was a cross-sectional, descriptive study, carried out from April 1 to May 31, 2021 at the hospitals of Kossodo and Boulmiougou in Ouagadougou. Data collection was done using an anonymous, pre-established self-administered questionnaire. Results: A total of 246 caregivers were participated in the study. The average age was 44.8 years and 77.2% had more than 10 years of seniority in the function. The existence of the anti-tobacco law in Burkina Faso in public places was known by 78.9% of caregivers. Most caregivers were aware of tobacco-related harm (93.5%). The most known tobacco-related pathologies were lung cancer (55.3%), throat cancer (13.8%). Posters (88.2%), the ban on smoking in hospitals (93.9%), staff training (80.5%) and monitoring of compliance with the anti-smoking law (46%) were the major means recommended to achieve a tobacco-free hospital. Conclusion: Caregivers should serve as role models in tobacco control. The establishment of an anti-tobacco surveillance committee in health structures and the strengthening of awareness are to be promoted.