Intramuscular injections can be life threatening when applied to the wrong area. For this reason, safe areas should be preferred for injection application. This study is to evaluate the effectiveness of intramuscular injection training in the ventrogluteal region (VG) given to nursing students. The research was conducted with a single group using a semi-empirical pre-test, post-test design. The population of the study was composed of the senior nursing students (N=70) studyi ng in Nursing Department of University. The data were collected with survey method. The students were given theoretical and skill training about the VG region, and the students’ knowledge, opinions and preference about the ventrogluteal region were questioned. Descriptive statistical methods and Wilcoxon test in the evaluation of opinions about VG region before and after the training were used. It was determined that the prejudices of the students about injection to VG region changed significantly and positively after the training (90.5%) and their information level increased (p<0.05), but the students had negative thoughts about their self-confidence and about the possible objection of the patients (p>0.05). After the training, 8 students preferred the VG region, while 13 students applied injections to the dorsogluteal region. Although the knowledge level of the students about the VG region has increased and their prejudices have changed positively, there are students who prefer the DG region.