The present study consists in comparing pollination techniques in order to maintain the phenotypic characteristics of maize inbred lines. In fact, the objective of this work was to detect the influence of different pollination techniques on the maintenance of some characters of four maize inbred lines (i.e., REGN, CZL0919, MUL692 and CML312) in order to identify the most conservative multiplication technique of these inbred lines. To achieve this, two experiments were carried out. The first one consisted in maintaining the inbred lines according to the four techniques (selfing, SIB crossing, pollination with pollen bulk, and natural pollination in an isolated crossing block); and the second one consisted in the evaluation of their descendants (offspring) according to a split splot design with three replications at the Seed Production Center (CEPROSEM). The results of this study revealed that the selfing method presented the lowest genetic variance to the initial population. Therefore, this explains that the selfing method is the most conservative mode of multiplication. Notwithstanding, moving from one season to another, one should switch between self-fertilization (selfing) and the pollinisation with a bulk of pollen (half-sib), whether that being natural or artificial, to avoid the phenomenon of gene depression.