The rate of obesity has increased in Malaysia over the years and has become one of the leading causes of morbidity and mortality in the country. Nurses as role models have a professional responsibility to promote healthy lifestyle among the general population. This cross - sectional survey is aimed to determine the prevalence of obesity among female Registered Nurses in Malaysia, as well as to assess their medical and reproductive health statuses, eating patterns, exercise patterns, and stress reduction activities. Results revealed that most of the nurse-respondents were middle-aged, married, with more than 10 year experiences working as staff nurses in public hospitals. The BMI data shows that overweight and obesity is an actual and emerging issue among nurses. Out of the 1086 Registered Nurses, 50.6% were either pre-obese (33.5%) or obese (17.1%). Nurses and other stakeholders should address this problem promptly. There is indication of knowledge-behavior gap among the nurse-respondents' overall lifestyle patterns. Their knowledge in terms of proper dietary choices, rest, and stress reduction activities needs to be reinforced to promote healthier lifestyle. It is recommended that staff health especially the monitoring of weight be included as part of health promotional programs initiative of every organization together with a supportive environment. Attention must also be given to work-related conditions which has an impact on stress.