The present study has been conducted in Babylon/Science College for Women to detect the efficiency of three plant extracts (Euphorbia helioscobia, Eucalyptus globules and Myrtus comminus on the Entamoeba histolytica Parasites that grown in beef liver infusion medium at 37 centigreat temperature. Concentration of cooled and boiled plant extracts its(2.5 ,5 , 10) % of the three plants as well as control group(distelled water). The results revealed that the boiled plant extracts of three plants were the efficient from cooled plant extracts in killing the trophozoites of the Entamoeba histolytica . The E. helioscobia boiled plant extracts was the efficient to killing the trophozoites in the second day from the begining of the experiment with 10 mgml. And the next its boiled plant extracts of Eucalyptus globules and Myrtus comminus in the third day from the begining of the experiment with 5 mgml for each one .and all the three plant extracts inhibited the viability of E. histolytica that grown in beef liver infusion medium comparison with control group.as well as the metronidazole drug in one microliterml. finished the viability of trophozoites at the third day, and showed from the present study successfully grown E. histolytica in beef liver infusion medium.