This study aimed to determine the characteristics of antibiotic resistance in strains of Escherichia coli isolated from lettuce. 3 vegetable crop production sites were randomly selected. A total of 60 lettuce samples were taken from 20 lettuce plants randomly collected per site. Escherichia coli strains were isolated on Rapid E.coli 2 medium and their identification confirmed on API 20E gallery. Then, an antibiotic susceptibility test using the disk diffusion method in Müller-Hinton agar medium was carried out on the identified strains. The results revealed relatively high resistance rates to amoxicillin (73.5%, 66.5% and 62.5% at sites 1, 2 and 3 respectively) and tetracycline (86% for site 1, 67% for site 2 and 79.5% for site 3). The proportions of expanded-spectrum betalactamase were 22.5% for site 1, 44.5% for site 2, and 56% for site 3. A total of 246 extended-spectrum betalactamase-producing strains were observed out of 600 strains isolated, i.e. 41%. The resistance genes BlaCTXM, BlaSHV and BlaTEM were observed in the respective proportions of 32.5%, 43.1% and 56.1% in extended-spectrum betalactamase-producing strains. These antibiotic-resistant strains of Escherichia coli could constitute a health hazard for humans, because lettuces are vegetables eaten raw.

Edible frogs Hoplobatrachus occipitalis represent an animal protein source for most population of West area of Côte d’Ivoire, whose sanitary quality are understand. The objectives of this study was to determinate the rate of Aeromonas sp. from these frogs and to evaluate in vitro potential virulence of the strains. A total of 300 frog including 210 fresh and 90 smoked from three markets were collected. The analyses of samples consisted to isolation of Aeromonas, the biotypage and the determination of in vitro virulence factors. The results reveal that no smoked frog is contaminated; on other hand, the percentage of contamination of fresh frog is 23.0% with the prevalence of 70.5%. Species Aeromonas hydrophila (76.4 %) and Aeromonas sobria (23.6%) have been exclusively identified. The biotype of Aeromonas hydrophila characterized by no utilization of mannose and citrate was most frequent (41.7%). The determinants of the virulence of strains are the production of hemolysin (70.1%), protease (68.9%), ADNase (97.3%), lipase (41.1%) and amylase (55.4%). The high prevalence of Aeromonas with a potential pathogen from edible frog, represent a risk for consumers and require the measure of assurance for the sanitary safety of products.

This study aimed to reduce the risk of consumption of atti