Volume 8, Issue 1, September 2014, Pages 185–194
Aamer Bin Zahur1, Hamid Irshad2, Asma Latif3, Aman Ullah4, Riasat Wasee Ullah5, Muhammad Afzal6, Sarosh Majid Salaria7, Zafar-ul-Ahsan Qureshi8, Khawar Mahboob9, and Shahida Afzal10
1 Animal Health Research Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan
2 Animal Health Research Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan
3 Animal Health Research Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan
4 Animal Health Research Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan
5 Animal Health Research Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan
6 Progressive Control of PPR in Pakistan, FAO Pakistan (GCP/PAK/127/USA), Pakistan
7 Azad Jammu and Kashmir Medical College, Muzaffarabad, Azad Kashmir, Pakistan
8 Veterinary Research Institute, Lahore, Pakistan
9 Veterinary Research Institute, Lahore, Pakistan
10 Veterinary Research Institute, Lahore, Pakistan
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
A total of 62 Peste des Petits ruminants (PPR) outbreaks in sheep and goat flocks were investigated in Pakistan during 2005-2007. The presence of PPR virus (PPRV) was confirmed by clinical picture, necropsy examination, Ic-ELISA, virus isolation and RT-PCR. Of 397 tissue samples, 65% tested positive by Ic-ELISA. Six PPR virus isolates were obtained through cell culture on VERO or GKC cell from 61Ic-ELISA positive samples identified by characteristic CPEs and confirmed by testing the cell culture supernatant by Ic-ELISA and RT-PCR using PPRV specific F gene based primers. The sequence data of F gene from 6 isolates was analyzed for identities and a phylogenetic tree was generated based on 372bp F gene sequences of PPRV. The isolates were clustered into lineage 4 along with other Asian isolates. The recent isolates and a previous isolate from Pakistan (PAK-2004) were found to be monophyletic having close relationship with an Indian isolate (IND-PON).
Author Keywords: PPR, isolation, Pakistan, phylogenetic analysis, Ic-ELISA.
Aamer Bin Zahur1, Hamid Irshad2, Asma Latif3, Aman Ullah4, Riasat Wasee Ullah5, Muhammad Afzal6, Sarosh Majid Salaria7, Zafar-ul-Ahsan Qureshi8, Khawar Mahboob9, and Shahida Afzal10
1 Animal Health Research Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan
2 Animal Health Research Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan
3 Animal Health Research Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan
4 Animal Health Research Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan
5 Animal Health Research Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan
6 Progressive Control of PPR in Pakistan, FAO Pakistan (GCP/PAK/127/USA), Pakistan
7 Azad Jammu and Kashmir Medical College, Muzaffarabad, Azad Kashmir, Pakistan
8 Veterinary Research Institute, Lahore, Pakistan
9 Veterinary Research Institute, Lahore, Pakistan
10 Veterinary Research Institute, Lahore, Pakistan
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
A total of 62 Peste des Petits ruminants (PPR) outbreaks in sheep and goat flocks were investigated in Pakistan during 2005-2007. The presence of PPR virus (PPRV) was confirmed by clinical picture, necropsy examination, Ic-ELISA, virus isolation and RT-PCR. Of 397 tissue samples, 65% tested positive by Ic-ELISA. Six PPR virus isolates were obtained through cell culture on VERO or GKC cell from 61Ic-ELISA positive samples identified by characteristic CPEs and confirmed by testing the cell culture supernatant by Ic-ELISA and RT-PCR using PPRV specific F gene based primers. The sequence data of F gene from 6 isolates was analyzed for identities and a phylogenetic tree was generated based on 372bp F gene sequences of PPRV. The isolates were clustered into lineage 4 along with other Asian isolates. The recent isolates and a previous isolate from Pakistan (PAK-2004) were found to be monophyletic having close relationship with an Indian isolate (IND-PON).
Author Keywords: PPR, isolation, Pakistan, phylogenetic analysis, Ic-ELISA.
How to Cite this Article
Aamer Bin Zahur, Hamid Irshad, Asma Latif, Aman Ullah, Riasat Wasee Ullah, Muhammad Afzal, Sarosh Majid Salaria, Zafar-ul-Ahsan Qureshi, Khawar Mahboob, and Shahida Afzal, “Isolation and characterization of lineage-IV Peste des Petits Ruminants (PPR) virus strains from Pakistan,” International Journal of Innovation and Applied Studies, vol. 8, no. 1, pp. 185–194, September 2014.
