Volume 7, Issue 4, August 2014, Pages 1292–1297
Trébissou Jonhson Noel David1, Yavo William2, Tano Konan Dominique3, Dablé Trésor Maruis4, Amiah Ahou Mireille5, N'cho Monsan6, and Djaman Allico Joseph7
1 Pharmacodynamics Biochemical Laboratory, UFR Biosciences, Felix Houphouet Boigny University, PO BOX, 582 Abidjan 22, Cote d'Ivoire
2 Laboratory of Microbiology National of Public Health (INSP) of Cote d'Ivoire, Cote d'Ivoire
3 Laboratory of Microbiology National of Public Health (INSP) of Cote d'Ivoire, Cote d'Ivoire
4 Laboratory of Microbiology National of Public Health (INSP) of Cote d'Ivoire, Cote d'Ivoire
5 Laboratory of Microbiology National of Public Health (INSP) of Cote d'Ivoire, Cote d'Ivoire
6 Laboratory of Microbiology National of Public Health (INSP) of Cote d'Ivoire, Cote d'Ivoire
7 Pharmacodynamics Biochemical Laboratory, UFR Biosciences, Felix Houphouet Boigny University, PO BOX, 582 Abidjan 22, Côte d’Ivoire
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
To evaluate the in vitro chemosensitivity to Atovaquone (ATQ) and analyze polymorphism of Pfcytb gene of Plasmodium falciparum isolates from Abidjan. Fifty seven isolates of P. falciparum collected between 2010 and 2012 from the outskirts of the city of Abidjan. Were used for testing in vitro susceptibility according to the microtest optical version of the World Health Organization (WHO). Polymerase Chain Reaction (PCR) was used to determine mutation in the Pfcytb gene. The analysis of the relationship between the observed mutations and chemosensitivity of isolates was made using Cohen's kappa test. The geometric mean IC50 of atovaquone is 1,645 mM. All 57 isolates were sensitive to atovaquone. The 29 isolates of P. falciparum sequenced showed no mutation of Pcytb gene at codon Y268. No other mutation was observed. Our study showed no correlation between phenotypic and molecular data (K< 0). This study shows that the combination atovaquone / proguanil can be one of the drugs of choice for the prophylaxis of multidrug-resistant falciparum malaria recommended by the World Health Organization (WHO).
Author Keywords: Plasmodium falciparum, Pfcytb, Atovaquone, In vitro susceptibility, C.
Trébissou Jonhson Noel David1, Yavo William2, Tano Konan Dominique3, Dablé Trésor Maruis4, Amiah Ahou Mireille5, N'cho Monsan6, and Djaman Allico Joseph7
1 Pharmacodynamics Biochemical Laboratory, UFR Biosciences, Felix Houphouet Boigny University, PO BOX, 582 Abidjan 22, Cote d'Ivoire
2 Laboratory of Microbiology National of Public Health (INSP) of Cote d'Ivoire, Cote d'Ivoire
3 Laboratory of Microbiology National of Public Health (INSP) of Cote d'Ivoire, Cote d'Ivoire
4 Laboratory of Microbiology National of Public Health (INSP) of Cote d'Ivoire, Cote d'Ivoire
5 Laboratory of Microbiology National of Public Health (INSP) of Cote d'Ivoire, Cote d'Ivoire
6 Laboratory of Microbiology National of Public Health (INSP) of Cote d'Ivoire, Cote d'Ivoire
7 Pharmacodynamics Biochemical Laboratory, UFR Biosciences, Felix Houphouet Boigny University, PO BOX, 582 Abidjan 22, Côte d’Ivoire
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
To evaluate the in vitro chemosensitivity to Atovaquone (ATQ) and analyze polymorphism of Pfcytb gene of Plasmodium falciparum isolates from Abidjan. Fifty seven isolates of P. falciparum collected between 2010 and 2012 from the outskirts of the city of Abidjan. Were used for testing in vitro susceptibility according to the microtest optical version of the World Health Organization (WHO). Polymerase Chain Reaction (PCR) was used to determine mutation in the Pfcytb gene. The analysis of the relationship between the observed mutations and chemosensitivity of isolates was made using Cohen's kappa test. The geometric mean IC50 of atovaquone is 1,645 mM. All 57 isolates were sensitive to atovaquone. The 29 isolates of P. falciparum sequenced showed no mutation of Pcytb gene at codon Y268. No other mutation was observed. Our study showed no correlation between phenotypic and molecular data (K< 0). This study shows that the combination atovaquone / proguanil can be one of the drugs of choice for the prophylaxis of multidrug-resistant falciparum malaria recommended by the World Health Organization (WHO).
Author Keywords: Plasmodium falciparum, Pfcytb, Atovaquone, In vitro susceptibility, C.
How to Cite this Article
Trébissou Jonhson Noel David, Yavo William, Tano Konan Dominique, Dablé Trésor Maruis, Amiah Ahou Mireille, N'cho Monsan, and Djaman Allico Joseph, “In Vitro Susceptibility to Atovaquone and Characterization of Pfcytb Gene of Plasmodium falciparum Isolates From Abidjan (Côte d'Ivoire),” International Journal of Innovation and Applied Studies, vol. 7, no. 4, pp. 1292–1297, August 2014.
