The study aims to assess the cutaneous toxicity and antifungal activity of the hydroethanol extract of Senna podocarpa, a plant used in traditional medicine. This research is essential to determine both the safety of using the extract on the skin and its efficacy against various fungal infections. Following OECD guideline 404 (2015), twelve Hyplus rabbits were treated with 200 mg/kg and 500 mg/kg doses of the extract to observe skin reactions, such as erythema and oedema, over 14 days. Antifungal activity was assessed using the double dilution slant tube method, followed by inoculation with Candida albicans, Aspergillus fumigatus, and Trichophyton mentagrophytes. Antifungal parameters such as MIC, MFC, and IC50 were determined. Senna podocarpa extract showed no dermal toxicity, with a mean irritation index (MII) of 0, indicating that it is neither irritant nor corrosive to rabbit skin. No skin lesions were observed, and the rabbits’ coats grew back 24 hours after application. In addition, the extract did not affect the weight of the rabbits, with those given 500 mg/kg even showing greater weight gain than those given 200 mg/kg. In terms of antifungal activity, the extract inhibited the growth of the fungi tested in a dose-dependent manner. The MIC and MFC were 6.25 mg/mL and 12.5 mg/mL for A. fumigatus, 25 mg/mL and 100 mg/mL for C. albicans, and 100 mg/mL for T. mentagrophytes.

Plant-based alcoholic mixtures are widely appreciated by the Ivorian population due to their low cost. The aim of this study was to assess the safety of extractible contained in one of these mixtures («Plaie de ventre») sold for its health claims in the commune of Yopougon (Côte d’Ivoire). A consumption survey was carried out on these alcoholic mixtures using a questionnaire. Subsequently, an acute toxicity study was carried out on the consumers’ favorite drink by administering the dry extractible from this mixture to three batches of rats at doses of 500, 2500 and 5000 mg/kg bw. Animals were observed for 14 days for clinical signs of intoxication, and hematological and biochemical parameters were assayed. The results of this study revealed that mixture «Plaie de ventre» was the drink preferred by consumers (53.33%). Moreover, administration of the extractible from this mixture revealed no behavioral changes in the rats, and estimated LD50 was greater than 5000 mg/kg bw. In addition, evaluation of hematological and biochemical parameters revealed a significant increase (p < 0.05) in white blood cell count, blood platelet count, and serum ASAT and ALAT levels. Thus, extractible from mixture «Plaie de ventre» constitute a health risk for consumers.

The objective of this study was to perform triphytochemistry and to evaluate the effect of aqueous (AqE) and hydroethanol (EthE) extracts of Spathodeae campanulata on blood glucose and pancreatitis markers in streptozotocin (STZ) -induced diabetic male rats. S. campanulata P. beauv, is a plant belonging to the Bignoniaceae family which is traditionally used for the treatment of diabetes, malaria and schistosomiasis. In a first step, we performed triphytochemistry of the extracts which showed that the aqueous and hydroethanolic extracts of the barks are rich in polyphenols, flavonoids, saponosides, alkaloids, sterol-polyterpenes, tannins but relatively poor in quinones. In a second phase, this study consisted in evaluating the effects on glycaemia and markers of pancreatitis of AqE and EthE of S. campanulata administered to 46 male rats of the Wistar strain divided into 9 batches of four male rats made diabetic each by intraperitoneal injection of a single dose of 60 mg/kg/bw of STZ. After 28 days of treatment with 200, 400 and 800 mg/kg/bw of S. campanulata AqE and EthE, blood glucose, alpha amylase and lipase activities were significantly decreased with both extracts.

The aim of this study was to develop an ointment of cosmetic interest in order to offer populations a hair product with local ingredients, a study of formulation and conditioning of the ointment was undertaken using oils of Elæis guineensis as active and of Butyrospermum. parkii as an excipient. These two species are known in communities for their use in hair maintenance and growth. The formulation consisted of a mixture of these two oils in precise proportions to obtain a single homogeneous phase. The ointment obtained coded PKP was applied to male and female rabbits of the species Oryctolagus cuniculus previously shaved at the rate of two applications per day for 28 days. Hair growth was measured each two days. In addition, sterility, stability and skin tolerability were assessed. The PKP ointment obtained was moderately hard, homogeneous, with a very pleasant odor and a pH of 3.93. After 28 days of experimentation, the results showed that hair growth in male rabbits was greater with a maximum cumulative length of 21 mm. The ointment did not show any microbial contamination and it remained stable over time. PKP is an ointment that would stimulate hair growth and would be a very good alternative to fight against early hair loss.

We studied the cutaneous MISCA-MATES toxicity soap humans. The test of irritation at the man is indispensable to specify the potential irritating power of some better cosmetic preparations. Thirty soaps of MISCA-MATÈS experimentation were delayed on thirty voluntary people. Two methods have been used. The first consists in applying a quantity of soap foam on the hand of the volunteers during 24 hours. The test has been repeated at the same place during 7 days except if an erythema develops in which case the test is stopped. The treatment has not been interrupted after the 7 days of experimentation. The second method consists in distributing to each volunteer a soap for the bath during 7 days and we noted the effects which could produce the soap on their respective skins. The analysis shows that one person out of thirty (3.33 %) felt a slight burn when applying soap while twenty nine persons (96.67 %) felt no burn.

Chloroquine has been widely used in the treatment of uncomplicated Plasmodium falciparum malaria. Unfortunately, because of chemoresistance emergence and its spread worldwide since the 1960's, substitute medicines were compulsory to fight against malaria efficiently.
Therefore, current priority of the fight against malaria is to search for substances capable to improve back chloroquine efficacy.
The present work highlights life threatening action of Olax subscorpio

To evaluate the in vitro chemosensitivity to Atovaquone (ATQ) and analyze polymorphism of Pfcytb gene of Plasmodium falciparum isolates from Abidjan. Fifty seven isolates of P. falciparum collected between 2010 and 2012 from the outskirts of the city of Abidjan. Were used for testing in vitro susceptibility according to the microtest optical version of the World Health Organization (WHO). Polymerase Chain Reaction (PCR) was used to determine mutation in the Pfcytb gene. The analysis of the relationship between the observed mutations and chemosensitivity of isolates was made using Cohen's kappa test. The geometric mean IC50 of atovaquone is 1,645 mM. All 57 isolates were sensitive to atovaquone. The 29 isolates of P. falciparum sequenced showed no mutation of Pcytb gene at codon Y268. No other mutation was observed. Our study showed no correlation between phenotypic and molecular data (K< 0). This study shows that the combination atovaquone / proguanil can be one of the drugs of choice for the prophylaxis of multidrug-resistant falciparum malaria recommended by the World Health Organization (WHO).