[ دراسة وبائية مصلية لمرضى السكري المصابين بداء المقوسات في محافظة بابل ]
Volume 12, Issue 1, July 2015, Pages 183–189
Najma Ali sharad1 and Ahmed Khudhair AL-Hamairy2
1 Biology, Babylon/Science College for Women, Al-Hilla, Babylon Province, Iraq
2 Biology, Babylon/Science College for Women, Al-Hilla, Babylon Province, Iraq
Original language: Arabic
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Identification and seroepidemiological and molecular study were conducted for diabetic mellitus patients to investingate the infected person with Toxoplasmosis in the Babylon province for two sexes during the period from October 2013 till July 2014. Latex agglutination (LA) tests were used for 550 serum samples and ELISA technique for 350 serume samples (where positive with LA test) and polymerase chain reaction (PCR) technique for 100 blood samples (where positive with ELISA technique), according to epidemiological criterions (Sex , area region , age group , educational level , presence or absence of animals and presence or absence of other companion diseases). The results showed that the total toxoplasmosis samples positive using the test latex (63.6%) and ELISA antibody IgG (51.4%) and antibody IgM (% 11.4). polymerase chain reaction technique was (45%) and the results showed that there were significant differences between males and females for tests the above three, the housing area for testing latex the city were more prone to infection (65.1%) The ELISA test was the city more susceptible to infection (53.4%) for antibody IgG were districts and suburban more vulnerable to infection (9.7%) for the antibody IgM.
Author Keywords: Toxoplasmosis, diabetic mellitus, Latex agglutination, ELISA technique, PCR technique.
Volume 12, Issue 1, July 2015, Pages 183–189
Najma Ali sharad1 and Ahmed Khudhair AL-Hamairy2
1 Biology, Babylon/Science College for Women, Al-Hilla, Babylon Province, Iraq
2 Biology, Babylon/Science College for Women, Al-Hilla, Babylon Province, Iraq
Original language: Arabic
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Identification and seroepidemiological and molecular study were conducted for diabetic mellitus patients to investingate the infected person with Toxoplasmosis in the Babylon province for two sexes during the period from October 2013 till July 2014. Latex agglutination (LA) tests were used for 550 serum samples and ELISA technique for 350 serume samples (where positive with LA test) and polymerase chain reaction (PCR) technique for 100 blood samples (where positive with ELISA technique), according to epidemiological criterions (Sex , area region , age group , educational level , presence or absence of animals and presence or absence of other companion diseases). The results showed that the total toxoplasmosis samples positive using the test latex (63.6%) and ELISA antibody IgG (51.4%) and antibody IgM (% 11.4). polymerase chain reaction technique was (45%) and the results showed that there were significant differences between males and females for tests the above three, the housing area for testing latex the city were more prone to infection (65.1%) The ELISA test was the city more susceptible to infection (53.4%) for antibody IgG were districts and suburban more vulnerable to infection (9.7%) for the antibody IgM.
Author Keywords: Toxoplasmosis, diabetic mellitus, Latex agglutination, ELISA technique, PCR technique.
Abstract: (arabic)
أجريت دراسة وبائية مصلية وجزيئية للتحري عن داء المقوسات لدى مرضى السكري بالسكري ولكلا الجنسين في محافظة بابل للمدة من تشرين الاول 2013 ولغاية تموز2014 , أستعملت بالدراسة أختبار التلازن المباشر (اللاتكس) لما مجموعة 550 عينة مصل , وأختبار الاليزا 350 عينة مصل (موجبة بأختبار اللاتكس) وتقنية تفاعل سلسلة متعدد البلمرة 100 عينة دم (موجبة بأختبار الأليزا) وأعتمدت المعايير الوبائية (الجنس ,منطقة السكن ,الفئة العمرية ,المستوى التعليمي , وجود الحيوانات من عدم وجودها ووجود أمراض أخرى مرافقة). أظهرت النتائج أن الاصابة الكلية بداء المقوسات للعينات الموجبة بأستعمال أختبار اللاتكس (63.6%) وأختبار الاليزا للضد ) 51.4%) IgG و للضد (%11.4) IgM تقنية تفاعل سلسلة متعدد البلمرة بمقدار (45%) وأظهرت النتائج وجود فروقات معنوية بين الذكور والاناث بالنسبة للاختبارات الثلاثة انفة الذكر, أما منطقة السكن بالنسبة لاختبار اللاتكس فكانت المدينة أكثر عرضة للاصابة (65.1 %) أما أختبار الاليزا فكانت المدينة أكثر عرضة للاصابة (53.4%) بالنسبة للضد IgG وكانت الأقضية والنواحي أكثر عرضة للأصابة (9.7%) .IgM
Author Keywords: داء المقوسات ، داء السكري ، فحص اللاتكس ، فحص اللاليزا، تقنية تفاعل سلسلة متعدد البلمرة.
How to Cite this Article
Najma Ali sharad and Ahmed Khudhair AL-Hamairy, “Seroepidemiological study for patients with diabetes that infected with Toxoplasma gondii in the Babylon province,” International Journal of Innovation and Applied Studies, vol. 12, no. 1, pp. 183–189, July 2015.
