Identification and seroepidemiological and molecular study were conducted for diabetic mellitus patients to investingate the infected person with Toxoplasmosis in the Babylon province for two sexes during the period from October 2013 till July 2014. Latex agglutination (LA) tests were used for 550 serum samples and ELISA technique for 350 serume samples (where positive with LA test) and polymerase chain reaction (PCR) technique for 100 blood samples (where positive with ELISA technique), according to epidemiological criterions (Sex , area region , age group , educational level , presence or absence of animals and presence or absence of other companion diseases). The results showed that the total toxoplasmosis samples positive using the test latex (63.6%) and ELISA antibody IgG (51.4%) and antibody IgM (% 11.4). polymerase chain reaction technique was (45%) and the results showed that there were significant differences between males and females for tests the above three, the housing area for testing latex the city were more prone to infection (65.1%) The ELISA test was the city more susceptible to infection (53.4%) for antibody IgG were districts and suburban more vulnerable to infection (9.7%) for the antibody IgM.