[ Caractérisation phénotypique des souches de staphylocoques antibiorésistantes isolées des voies uro-génitales et sensibilité aux alicaments (Brassica oleracea, Allium cepa, Allium schoenoprasum, Allium sativum et le Miel) à Kisangani (Province de la Tshopo, RD Congo) ]
Volume 43, Issue 1, July 2024, Pages 1–13
Jules Lokonga Okenge1, Landry Musole Ntwali2, and Sylvain Kumba Lubemba3
1 Département des sciences Biotechnologiques, Faculté des sciences, B.P. 2012, Université de Kisangani, RD Congo
2 Département des sciences Biotechnologiques, Faculté des sciences, B.P. 2012, Université de Kisangani, RD Congo
3 Département des sciences Biotechnologiques, Faculté des sciences, B.P. 2012, Université de Kisangani, RD Congo
Original language: French
Copyright © 2024 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The objective of this study was to phenotypically characterize antibiotic-resistant strains of staphylococci isolated from the urogenital tract and to test in vitro their sensitivity to extracts of medicinal products (Brassica oleracea; Allium cepa; Allium schoenoprasum, Alluim sativum and Honey). The concentrated crude extracts were obtained by the reflux method in which the juice obtained after pressing and filtering. Ethanolic and ethereal extracts obtained respectively by the extraction method using 95% ethanol and petroleum ether. The study of the sensitivity of the strains to the extracts was carried out by the method of diffusion in specific agar (Müller Hinton) on a petri dish containing discs soaked in solutions of the extracts. At the end of the experiments carried out, the following results were obtained: It emerges from the phenotypic characterization (morphological and biochemical) that all the strains of staphylococci studied are in clump and gram positive shell form. They all produced coagulase and catalase. Therefore, they all belong to the species S. aureus. The study of the sensitivity of the strains to different extracts of medicinal products reveals that the ethereal extracts are more effective than the concentrated and ethanolic crude extracts. They showed antibacterial activity in the majority of the strains tested. The largest inhibition diameter being in the order of 30 mm, this was achieved with the ethereal extract of B. oleracea. Concentrated ethanolic and crude extracts of B. oleracea respectively inhibited bacterial growth with a maximum diameter of around 17 and 18 mm. Honey exerted inhibitory activity of up to 17 mm in diameter. The strains were shown to be resistant to ethanolic extracts of A. cepa, A. schoenoprasum and A. sativum. This could be explained by the low concentration of the active ingredients in the solvents used and therefore the extracts were less likely to exert an effective inhibitory activity on the growth of antibiotic resistant strains of staphylococcus.
Author Keywords: Characterization, phenotypic, Staphylococcus, antibiotic resistance, uro-genital tract, sensitivity, food drugs.
Volume 43, Issue 1, July 2024, Pages 1–13
Jules Lokonga Okenge1, Landry Musole Ntwali2, and Sylvain Kumba Lubemba3
1 Département des sciences Biotechnologiques, Faculté des sciences, B.P. 2012, Université de Kisangani, RD Congo
2 Département des sciences Biotechnologiques, Faculté des sciences, B.P. 2012, Université de Kisangani, RD Congo
3 Département des sciences Biotechnologiques, Faculté des sciences, B.P. 2012, Université de Kisangani, RD Congo
Original language: French
Copyright © 2024 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The objective of this study was to phenotypically characterize antibiotic-resistant strains of staphylococci isolated from the urogenital tract and to test in vitro their sensitivity to extracts of medicinal products (Brassica oleracea; Allium cepa; Allium schoenoprasum, Alluim sativum and Honey). The concentrated crude extracts were obtained by the reflux method in which the juice obtained after pressing and filtering. Ethanolic and ethereal extracts obtained respectively by the extraction method using 95% ethanol and petroleum ether. The study of the sensitivity of the strains to the extracts was carried out by the method of diffusion in specific agar (Müller Hinton) on a petri dish containing discs soaked in solutions of the extracts. At the end of the experiments carried out, the following results were obtained: It emerges from the phenotypic characterization (morphological and biochemical) that all the strains of staphylococci studied are in clump and gram positive shell form. They all produced coagulase and catalase. Therefore, they all belong to the species S. aureus. The study of the sensitivity of the strains to different extracts of medicinal products reveals that the ethereal extracts are more effective than the concentrated and ethanolic crude extracts. They showed antibacterial activity in the majority of the strains tested. The largest inhibition diameter being in the order of 30 mm, this was achieved with the ethereal extract of B. oleracea. Concentrated ethanolic and crude extracts of B. oleracea respectively inhibited bacterial growth with a maximum diameter of around 17 and 18 mm. Honey exerted inhibitory activity of up to 17 mm in diameter. The strains were shown to be resistant to ethanolic extracts of A. cepa, A. schoenoprasum and A. sativum. This could be explained by the low concentration of the active ingredients in the solvents used and therefore the extracts were less likely to exert an effective inhibitory activity on the growth of antibiotic resistant strains of staphylococcus.
Author Keywords: Characterization, phenotypic, Staphylococcus, antibiotic resistance, uro-genital tract, sensitivity, food drugs.
Abstract: (french)
Caractérisation phénotypique des souches de staphylocoques antibiorésistantes isolées des voies Uro-génitales et sensibilité aux alicaments (Brassica oleracea; Allium cepa; Allium schoenoprasum; Allium sativum et le Miel) à Kisangani (RD. Congo). Cette étude a eu comme objectif de caractériser phénotypiquement des souches de staphylocoques antibiorésistantes isolées des voies uro-génitales et de tester in vitro leurs sensibilités aux extraits d’alicaments (Brassica oleracea; Allium cepa; Allium schoenoprasum, Alluim sativum et Miel). Les extraits bruts concentrés ont été obtenus par la méthode de reflux dans laquelle le jus était obtenu après pressage et filtrage. Les extraits éthanoliques et éthérés ont été obtenus par la méthode d’extraction respectivement à l’aide de l’éthanol à 95% et de l’éther de pétrole. La sensibilité des souches aux extraits a été étudiée par la méthode de diffusion en gélose spécifique (Müller Hinton) sur boîte de Pétri. A l’issu des expériences menées, les résultats suivants ont été obtenus: Il ressort de la caractérisation phénotypique (morphologique et biochimique) que toutes les souches de staphylocoques étudiées sont de forme coque en amas et gram positif. Elles ont toutes produits de la coagulase et de la catalase. De ce fait, elles appartiennent toutes à l’espèce Staphylococcus aureus. L’étude de la sensibilité des souches aux différents extraits d’alicaments révèle que les extraits éthérés sont plus efficaces que les extraits bruts concentrés et éthanoliques. Ils ont montré une activité antibactérienne chez la majorité des souches testées. Le diamètre d’inhibition le plus élevé étant de l’ordre de 30 mm, ceci a été obtenu avec l’extrait éthéré de B. oleracea. Les extraits éthanoliques et bruts concentrés de B. oleracea ont respectivement inhibé la croissance bactérienne avec un diamètre maximum de l’ordre de 17 et de 18 mm. Le Miel a exercé de sa part une activité inhibitrice avec un diamètre maximum de 17 mm. Les souches se sont montrées résistantes aux extraits bruts concentrés éthanoliques des A. cepa, A. schoenoprasum et A. sativum. Ceci pourrait s’expliquer par la faible concentration des principes actifs dans les solvants utilisés et par conséquent, les extraits ont été moins susceptibles d’exercer une activité inhibitrice efficace sur la croissance des souches de staphylocoques antibiorésistantes.
Author Keywords: Caractérisation, phénotypique, Staphylocoque, antibiorésistantes, voies uro-génitales, sensibilité, alicaments.
How to Cite this Article
Jules Lokonga Okenge, Landry Musole Ntwali, and Sylvain Kumba Lubemba, “Phenotypic characterization of antibioresistant staphylococcals isolated from the Uro-genital tract and susceptibility to food drugs (Brassica oleracea, Allium cepa, Allium schoenoprasum, Allium sativum and Honey) in Kisangani (Tshopo Province, DR Congo),” International Journal of Innovation and Applied Studies, vol. 43, no. 1, pp. 1–13, July 2024.
