Blood Exposure Accidents (BEA) are a daily risk faced by the staff of Kolda’s Regional Hospital Center (RHCK).The objective of this study was to assess the prevalence of BEA within this structure and to identify their main determinants. We have done a cross-sectional, descriptive study for an analytical purpose carried out over a period of two months. A total of 81 professionals agreed to participate in the study. Forty of them were victims of BEA (49.3%). The average age of the victims was 36.7 years. Women were the most affected (65%). 45% of victims were previously vaccinated against hepatitis B, before the occurrence of the accident. Nurses were more numerous (42.5%) and maternity was the service where the most victims were collected (25%). The mechanism of occurrence of the accident was dominated by the bite (82.5%). The most common procedures involved were surgical suture (20%), injections (20%) and catheterization (20%). The reporting rate was 66.7%. No contamination was observed. Several risk factors for the occurrence of BEA have been identified. They are psychological (stress, tiredness), organizational (overloaded and/or perforated containers, overwork), environmental (lack of lighting, unsuitable premises). Other factors were related to the patients (agitations, emergencies). In addition to this, there is a lack of respect for standard precautions as well as a lack of training and awareness among staff. Conclusion: the study has shown the need to raise awareness, train and improve the working conditions of caregivers.