The present study aims to evaluate the diversity of ichthyological populations in the section of the Bandama River located in the Upper Bandama Fauna and Flora Reserve (RFF-HB). This study was carried out, following the longitudinal gradient (upstream-downstream) of the section of the Bandama River in the reserve, on 06 stations and during 08 sampling campaigns between January 2018 and February 2019. The collection of fish from the experimental fishery was carried out using gill nets, creels and hawks. The ichthyofauna inventory identified 33 fish species divided into 17 families and 8 orders. The fish populations are more diversified in the middle course (25 species) and upstream (24 species) of the reserve. The frequencies of occurrence of the ichthyofauna show that Labeo coubie, Brycinus imberi, Chrysichthys nigrodigitatus, Heterobranchus isopterus and Oreochromis niloticus are very frequently encountered on the Bandama river in the reserve. The analysis of the diversity of the ichthyological populations of the reserve shows that the fish populations are less diversified but more stable and balanced.