The abusive use of chemical fertilizer in vegetable farming deteriorates the quality of vegetables and fruits. The objective of this study was to evaluate the effect of biofertilizers (Azolla caroliniana filtrate and compost) on the quality of tomato fruits through physicochemical and organoleptic parameters. Experiments were conducted over two consecutive years with four fertilization treatments: Azolla caroliniana filtrate, sawdust-based compost, NPK (10-18-18), and a control without fertilization. For each experiment, the fruits were sampled after ripening and transported to the laboratory for physicochemical analyses. The quantitative descriptive method was used for the sensory analysis of the fruits through the opinions of a tasting panel composed of 60 people. The results showed that Azolla caroliniana induced an increase in the pH (4.31) of the fruits compared to other treatments. Similarly, the application of Azolla significantly increased the vitamin C content (44.44 mg/100 g.fw) and lycopene content (6.43 mg/100 g.fw) of the fruits. Furthermore, the fruits of plants fertilized with compost and Azolla caroliniana were judged to be less salty, firm, and red-colored by the majority of the tasters. These biofertilizers allow for the production of high-quality tomatoes. Therefore, they can be used as alternatives to chemical inputs for soil fertilization.