Abstract: This study aims to analyze the perception of producers on agro-ecological practices in cotton growing areas in Benin. To do this, data was collected from 300 producers in three municipalities (Banikoara, Sinendé and Djidja) using a semi-structured questionnaire digitized on Kobocollecte. The calculation of the response rates and the ascending hierarchical classification carried out using the statistical software Ri386.4.0.4, made it possible to arrive at three classes of agro-ecological practices according to the perception of the producers. These classes of practices are: lightly restrictive, moderately restrictive and very restrictive. The major constraints are organizational, material, climatic and institutional. The study recommends strengthening the supervision of producers and taking into account the socio-economic characteristics of producers in the promotion of agro-ecological practices in cotton-growing areas.