Macroscopic examination is a description of a tissue sample. This procedure involves observation of the specimen, together with the samples taken. As part of our laboratory’s ongoing quality approach, we carried out a study to assess the quality of macroscopic examination in pathological anatomy. The assessment and interviews carried out identified existing good practices and areas for improvement, namely: a lack of space under the fume hood, unsuitable bottles for certain specimens, communication difficulties with surgeons, staff exposure to chemical hazards, particularly formalin, and infectious risks when examining fresh anatomical specimens. The recommendations put forward in this study are aimed at improving this essential technique for accurate anatomical-pathological diagnosis.