This research is considering the relationship between internet service quality and using internet banking in branches of Sepah bank in Guilanprovince. Statisticalsociety of this research is customers of branches of Sepah bank in Guilan province that is unlimited. Statistical sample of this research was 384 people regarding Morgan table and unlimited society and sampling method of the research was simple random. Current research based on goal is applied and regarding method of data collection it is descriptive and correlation type. For determining validity of research variable content method and for reliability cronbach alpha correlation has been used. For data analysis firstly by using kolmogrov-smirnov test normality of data were considered. For testing hypothesis Pearson correlation has been used. The result of research showed that there is relationship between internet service quality and using internet banking. Also there is relationship between accessibility, certainty of implementation, responsibility, attraction of website with using internet banking.