The objective of this work is the identification of certain biomolecules such as tocopherols, sterols, alcohols, aliphatic and terpene of sesame oil (sesanum indicum l.) from Congo Brazzaville in the Department of the bowl to the village of ONTOGO. This oil contains tocopherol which are composed of: gamma tocopherol (Wgamma): 171 mg/kg to MG, followed Delta tocopherol (W delta): 5 mg/kg. There is a content of total sterols of 543 mg/kg to MG including Beta-sitosterol (58.9%) follow-up of Campesterol (16.7%) and Delta5-Avenasterol (10.7%) and two middle peaks that will be Delta5-Avenasterol (10.7%) and Stigmaterol (7.2%). alcohols present in sesame also contains the terpene alcohols which are: the important thing is that of lupeol (9.0%) followed the cycloartenal (4.4%) and finally the α-amyrin (1.3%) , and the aliphatic alcohols are forms of traces. All of these biomolecules are important in the prevention of cardiovascular disease for some and fight some cancers for others.