A novel nanoscale henna natural dye with particle size less than 100 nm were successfully prepared by using ultrasonic stirrer. Henna natural dye as ecofriendly dye was studied to clarify the impact of nature of nano-size color particles on size, shape, and particle distribution of the natural dye. The work was extended to study the K/S and overall color fastness properties of the printed natural fabrics (wool, silk and cotton) in presence and absence of mordant. Results showed that the K/S values of nano scale samples acquire higher values when compared to that of the original samples, irrespective of the nature of the fabric used. Mordant for example Alum that incorporated with original henna can be omitted, and substituted by nano-henna without mordant on printing silk and cotton fabrics. Results also shows that the pre-mordanting acquired K/S values higher than the simultaneous mordanting irrespective of the kind of fabric used, or dye particles size used, or henna concentrations. While on using tannic acid as a mordant, color fastness to rubbing, and perspiration properties of nano dye is found to be better than that of the original.