[ Inter comparaison des analyses des phycotoxines paralysantes (PSP) au niveau des coques rouges entre le laboratoire de la société DINAY et les laboratoires des phycotoxines de l'INRH réalisées par test biologique sur souris (protocole AOAC n°: 959.08, 1990) ]
Volume 7, Issue 4, August 2014, Pages 1344–1352
Dalila Ghallab1, Hassan Bouzidi2, Najat Oualit3, Mohammed Marhraoui4, Mouhamed Al maadoudi5, and Abdenbi Bendriss6
1 Université Abdelmalek Essaâdi, Faculté des Sciences, Tétouan, Maroc
2 Société DINAY 39, Zone Industrielle, Tétouan, Maroc
3 Société DINAY 39, Zone Industrielle, Tétouan, Maroc
4 Institut National de Recherche Halieutique – Station RSSL – M'diq, BP 31 Port Maritime de M'Diq, 93200 M'Diq, Tétouan, Maroc
5 Laboratoire Régional d'Analyse et Recherche Vétérinaire, Rue Ibn Toumart, BP N°3, Tanger, Maroc
6 Université Abdelmalek Essaâdi, Faculté des Sciences, Tétouan, Maroc
Original language: French
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The main vectors of paralytic shellfish poisoning are bivalves that accumulate toxins through their mode of nutrition (filtration) during proliferation of toxigenic algae.
Acanthocardia tuburculatum is one of the most important bivalve molluscs which cause paralytic shellfish poisoning problems in Moroccan and Spanish Mediterranean coasts this species is capable of accumulating high levels of PSP toxins for a long time in these same tissues when microalgae producing potentionnellement toxin are not present. This presents a danger to the health of consumers.
This work is part of a cooperation agreement and scientific research between the NHRI and DINAY Company is to do a study on the contamination of the red shell (Acanthocardia tuburculatum) paralytic marine biotoxins (PSP) by dosage of these last by the biological method in mice jointly by five laboratories.
Taking into account the coefficient of variation of the method of mouse bioassay is 20% our results concluded that the comparison of results obtained by the statistical test between the different laboratories revealed no significant difference in the same commission inter responsible for monitoring the safety of marine and shellfish acknowledged in September 2008 that these results are satisfactory.
This study also allowed taking measures during internal checks and monitoring of the marine environment as expand the batch of samples and diversifies in terms of size and that each lot is the subject of several repetitive analyzes.
Author Keywords: Acanthocardia tuberculatum, Paralytic Shellfish Poisoning (PSP), Mousse Bioassay, Morocco.
Volume 7, Issue 4, August 2014, Pages 1344–1352
Dalila Ghallab1, Hassan Bouzidi2, Najat Oualit3, Mohammed Marhraoui4, Mouhamed Al maadoudi5, and Abdenbi Bendriss6
1 Université Abdelmalek Essaâdi, Faculté des Sciences, Tétouan, Maroc
2 Société DINAY 39, Zone Industrielle, Tétouan, Maroc
3 Société DINAY 39, Zone Industrielle, Tétouan, Maroc
4 Institut National de Recherche Halieutique – Station RSSL – M'diq, BP 31 Port Maritime de M'Diq, 93200 M'Diq, Tétouan, Maroc
5 Laboratoire Régional d'Analyse et Recherche Vétérinaire, Rue Ibn Toumart, BP N°3, Tanger, Maroc
6 Université Abdelmalek Essaâdi, Faculté des Sciences, Tétouan, Maroc
Original language: French
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The main vectors of paralytic shellfish poisoning are bivalves that accumulate toxins through their mode of nutrition (filtration) during proliferation of toxigenic algae.
Acanthocardia tuburculatum is one of the most important bivalve molluscs which cause paralytic shellfish poisoning problems in Moroccan and Spanish Mediterranean coasts this species is capable of accumulating high levels of PSP toxins for a long time in these same tissues when microalgae producing potentionnellement toxin are not present. This presents a danger to the health of consumers.
This work is part of a cooperation agreement and scientific research between the NHRI and DINAY Company is to do a study on the contamination of the red shell (Acanthocardia tuburculatum) paralytic marine biotoxins (PSP) by dosage of these last by the biological method in mice jointly by five laboratories.
Taking into account the coefficient of variation of the method of mouse bioassay is 20% our results concluded that the comparison of results obtained by the statistical test between the different laboratories revealed no significant difference in the same commission inter responsible for monitoring the safety of marine and shellfish acknowledged in September 2008 that these results are satisfactory.
This study also allowed taking measures during internal checks and monitoring of the marine environment as expand the batch of samples and diversifies in terms of size and that each lot is the subject of several repetitive analyzes.
Author Keywords: Acanthocardia tuberculatum, Paralytic Shellfish Poisoning (PSP), Mousse Bioassay, Morocco.
Abstract: (french)
Les principaux vecteurs d'intoxication paralysante sont les mollusques bivalves qui accumulent les toxines par leur mode de nutrition (filtration) lors de prolifération des algues toxinogènes.
Acanthocardia tuburculatum est une des plus importants mollusques bivalves qui cause des problèmes d'intoxication paralysante dans les côtes méditerranéennes marocaines et espagnoles cette espèce est capable d'accumuler des taux importants de toxines paralysantes pendant longtemps dans ces tissus même lorsque les micro-algues produisant potentionnellement la toxine ne sont pas présentes. Ce qui présente un danger pour la santé des consommateurs.
Ce travail entre dans le cadre d'une convention de coopération et de recherche scientifique entre l'INRH et la société DINAY qui consiste à faire une étude sur la contamination de la coque rouge (Acanthocardia tuburculatum) par les biotoxines marines paralysantes (PSP) par dosage de ces derniers par la méthode biologique sur souris conjointement par cinq laboratoires.
En tenant compte que le coefficient de variation de la méthode de test biologique sur souris est de 20% nos résultats ont permis de conclure que la comparaison des résultats obtenus par le test statistique entre les différents laboratoires ne révèle pas de différence significative de même la commission interministérielle chargée du suivi de la salubrité du milieu marin et des coquillages a reconnu en septembre 2008 que ces résultats sont satisfaisantes.
Cette étude a permis aussi de prendre des mesures durant les autocontrôles et la surveillance de milieu marin telle que d'élargir le lot de prélèvement et le diversifier en terme de taille ainsi que chaque lot fait l'objet de plusieurs analyses répétitives.
Author Keywords: Acanthocardia tuberculatum, phycotoxines paralysantes (PSP), test souris, Maroc.
How to Cite this Article
Dalila Ghallab, Hassan Bouzidi, Najat Oualit, Mohammed Marhraoui, Mouhamed Al maadoudi, and Abdenbi Bendriss, “Inter-comparison analyzes of Paralytic Shellfish Poisoning (PSP) at the red shells from the laboratory of the company DINAY and laboratories phycotoxin INRH performed by mouse bioassay (AOAC protocol No.: 959.08, 1990),” International Journal of Innovation and Applied Studies, vol. 7, no. 4, pp. 1344–1352, August 2014.
