[ Impacts des pratiques phytosanitaires en milieu cotonnier sur le niveau de résistance phénotypique des populations de Anopheles gambiae (Diptera, culicidae) au Nord du Bénin ]
Volume 23, Issue 4, July 2018, Pages 365–372
Anges Yadouleton1, H. Wilfrid Djengue2, Michel SEZONLI3, B. Christophe CHABI4, Ramziyath Agbanrin5, Geraldo HOUNDETON6, Aboubacar SIDICK7, Fabrice AHISSOU8, and Martin AKOGBETO9
1 Centre de Recherche Entomologique de Cotonou, Benin
2 Laboratoire de Biotechnologies, Ressources Génétiques et Amélioration des Espèces Animales et Végétales (BIORAVE), Faculté des Sciences et Techniques de Dassa, Université Polytechnique d’Abomey, Benin
3 Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Benin
4 Département de Biochimie et Biologie Moléculaire, Faculté de Médecine, Université de Parakou, BP 123 Parakou, Benin
5 Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604 Cotonou, Benin
6 Centre de Recherche Entomologique de Cotonou, Benin
7 Centre de Recherche Entomologique de Cotonou, Benin
8 Centre de Recherche Entomologique de Cotonou, Benin
9 Centre de Recherche Entomologique de Cotonou, Benin
Original language: French
Copyright © 2018 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Insecticide treatments for crops protection in cotton fields have often been cited as the main factor in the selection of insecticide resistance in Anopheles gambiae populations. To explore this hypothesis further, the present study was designed to identify potential practices that may contribute to the emergence of insecticide resistance in Anopheles gambiae in cotton crop protection strategies such as the “Calendar Control Program (CCP)” and “Targeted Intermittent Control Program (TICP)” officially recommended already operate in Benin. Firstly, Knowledge Attitude-Practice (KAP) surveys were organized in the study sites to generate adequate information on the use of insecticide on cotton fields. In each site, leaders of farmer’s organizations were interviewed using semi-structured questionnaires that focused on the treatment strategies, and the use of insecticides in the farms. Further, bioassays were performed on adult mosquitoes collected from various of each program to assess the susceptibility of malaria vectors to insecticide-impregnated papers (permethrin 0.75%, delthamethrin 0.05%, DDT 4%, and bendiocarb 0.1%) following WHOPES guidelines. Results of this study showed that various pesticides particularly pyrethroids were used for pests control in CCP and TICP sites; ii)-Susceptibility tests showed that An. gambiae populations have developped resistance to DDT (4% as mean mortality despite the strategies), permethrin (30% in CCP and TICP), deltamethrin (38% and 46% as mean mortality in CCP and TICP respectivetly), but fully susceptible to bendiocarb . These findings confirmed the role of insecticide treatments in mosquitoes resistance. Measure must be taken for better management of the use of insectides for pest control.
Author Keywords: Anopheles gambiae, cotton, agricultural insecticide, resistance.
Volume 23, Issue 4, July 2018, Pages 365–372
Anges Yadouleton1, H. Wilfrid Djengue2, Michel SEZONLI3, B. Christophe CHABI4, Ramziyath Agbanrin5, Geraldo HOUNDETON6, Aboubacar SIDICK7, Fabrice AHISSOU8, and Martin AKOGBETO9
1 Centre de Recherche Entomologique de Cotonou, Benin
2 Laboratoire de Biotechnologies, Ressources Génétiques et Amélioration des Espèces Animales et Végétales (BIORAVE), Faculté des Sciences et Techniques de Dassa, Université Polytechnique d’Abomey, Benin
3 Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Benin
4 Département de Biochimie et Biologie Moléculaire, Faculté de Médecine, Université de Parakou, BP 123 Parakou, Benin
5 Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604 Cotonou, Benin
6 Centre de Recherche Entomologique de Cotonou, Benin
7 Centre de Recherche Entomologique de Cotonou, Benin
8 Centre de Recherche Entomologique de Cotonou, Benin
9 Centre de Recherche Entomologique de Cotonou, Benin
Original language: French
Copyright © 2018 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Insecticide treatments for crops protection in cotton fields have often been cited as the main factor in the selection of insecticide resistance in Anopheles gambiae populations. To explore this hypothesis further, the present study was designed to identify potential practices that may contribute to the emergence of insecticide resistance in Anopheles gambiae in cotton crop protection strategies such as the “Calendar Control Program (CCP)” and “Targeted Intermittent Control Program (TICP)” officially recommended already operate in Benin. Firstly, Knowledge Attitude-Practice (KAP) surveys were organized in the study sites to generate adequate information on the use of insecticide on cotton fields. In each site, leaders of farmer’s organizations were interviewed using semi-structured questionnaires that focused on the treatment strategies, and the use of insecticides in the farms. Further, bioassays were performed on adult mosquitoes collected from various of each program to assess the susceptibility of malaria vectors to insecticide-impregnated papers (permethrin 0.75%, delthamethrin 0.05%, DDT 4%, and bendiocarb 0.1%) following WHOPES guidelines. Results of this study showed that various pesticides particularly pyrethroids were used for pests control in CCP and TICP sites; ii)-Susceptibility tests showed that An. gambiae populations have developped resistance to DDT (4% as mean mortality despite the strategies), permethrin (30% in CCP and TICP), deltamethrin (38% and 46% as mean mortality in CCP and TICP respectivetly), but fully susceptible to bendiocarb . These findings confirmed the role of insecticide treatments in mosquitoes resistance. Measure must be taken for better management of the use of insectides for pest control.
Author Keywords: Anopheles gambiae, cotton, agricultural insecticide, resistance.
Abstract: (french)
Les traitements insecticides destinés à la protection de la culture du coton ont été souvent évoqués comme principal facteur de sélection de la résistance des populations d’Anopheles gambiae aux insecticides. Pour vérifier cette hypothèse, nous avons étudié le niveau de résistance phénotypique de An. gambiae s.l dans les zones cotonnières du Bénin selon deux programmes de protection contre les ravageurs : le programme calendaire avec utilisation de fortes quantités d’insecticide, le programme à lutte étagée ciblée (LEC) avec très peu d’insecticide. Dans un premier temps, notre enquête a porté sur la connaissance de la nature des pesticides utilisés contre les ravageurs de coton, leur origine et les doses appliquées. Ce qui nous a conduit à étudié la sensibilité des anophèles issus des sites d’étude aux papiers imprégnés de deltaméthrine (0,05%), de perméthrine (0,75%) de DDT (4%), et du bendiocarb (0,1%). Il ressort des investigations que plusieurs familles d’insecticides notamment les pyréthrinoïdes qui sont utilisés par les paysans au niveau des deux programmes. Cette utilisation de pesticides a entraîné une résistance de An. gambiae vis-à-vis du DDT (4% comme taux de mortalité moyenne), de la perméthrine (30%) et de la deltaméthrine (42%), mais reste sensible au bendiocarb (100%) quel que soit le programme. Cette étude confirme une fois encore que les traitements insecticides en agriculture sont responsables de la sélection de la résistance aux pyréthrinoïdes chez An. gambiae en Afrique de l’Ouest.
Author Keywords: Anophèle gambiae, coton, insecticides agricoles, résistance.
How to Cite this Article
Anges Yadouleton, H. Wilfrid Djengue, Michel SEZONLI, B. Christophe CHABI, Ramziyath Agbanrin, Geraldo HOUNDETON, Aboubacar SIDICK, Fabrice AHISSOU, and Martin AKOGBETO, “Impacts of cotton protection practices on the level of phenotypic resistance in Anopheles gambiae population in northern Benin,” International Journal of Innovation and Applied Studies, vol. 23, no. 4, pp. 365–372, July 2018.
