This study highlighted the symbiosis between Anabaena-azollae cyanobacteria and Azolla aquatic fern in the production of Azolla filiculoides and its use as a biofertilizer. His trial of vegetative production using ordinary water + dung, carried out in 14 days of culture, showed a significant amount (6473g). Then, the use of this one as an amendment for the growth of CB-one rice, showed its positive impact on soil fertility thus translating high yields of CB-one rice contrary to the unamended witness. Indeed, with this treatment, the average number of tillers observed after 56 days of culture, was 18.1 ± 4.23 tillers contrary to the NPK (15.83 ± 4.98) and the control (10, 4 ± 3.27). The average number of leaves was significant in Azolla (57.20 ± 13.45), average for the NPK fertilizer (48.86 ± 15.79) and low for the control (34.83 ± 14, 52). In sum, the trays treated with Azolla filiculoides produced many more panicles and grains compared to treatments. As a result, Azolla is likely to restore soil fertility by providing the soil with the nutrients needed for crop productivity.