In Morocco, some so called ‘‘forgotten fruits’’ such as the ‘‘Zizyphus Lotus’’ fruits and the ‘‘Arbutus Unedo’’ fruits can be valorized in the context of regional sustainable development as local products. In addition, there is a rapidly increasing prevalence of the urolithiasis in the world. The Urolithiasis involves the formation of crystalline aggregates called "urinary stones" that are developed in the urinary tract, usually in the kidneys or ureters, but may also affect the bladder or urethra. The aim of this study is to evaluate the in vitro anti-lithiasic activity of selected ‘‘forgotten fruits’’. The anti-lithiasic activity was evaluated against the aggregation of calcium oxalate. ‘‘Zizyphus Lotus’’ - and ‘‘Arbutus Unedo’’ fruits samples were taken from six zones from Beni Mellal-Khenifra region. The seed of the two fruits species were subjected to a grinding mortar. The samples have been subsequently, submitted a cold maceration using distilled water during 48 hours. The study of the crystallization of calcium oxalate has been carried out by the polarizing optical microscope (PLM). Some aqueous extracts have an anti-crystallization effect on the aqueous solution of the studied lithogenous species.