Night work in hospitals, despite its many negative psychosomatic, occupational and social repercussions, remains very little evaluated in African countries, and particularly in Senegal. The objective of this study was to evaluate its various repercussions on the health and socio-professional life of workers. For this, we conducted a cross-sectional and descriptive study over a period of 3 months using anonymous questionnaires. Our sample included health professionals performing night watch. A total of 65 had participated in the study. Women accounted for 63%. State nurses were more numerous (44.7%). The average age was 34.2 years old. The average seniority in night work was 7.1 years. 55.4% of workers felt comfortable with this type of schedule, compared with 44.6% feeling disrupted. 74.6% preferred daytime work. Several impacts of night work were identified. They were physiological, with poor sleep quality (48.4%), insufficient sleep duration (42.2%), menstrual disorders (24.7% of women). On the psychological level, stress (52.3%), temperamental disorders (26.5%), anxiety and depression (7.7%) were found. The somatic effects were chronic fatigue (100%), cardiovascular symptoms, weight disorders (46.1%). On a professional level, the nocturnal victims of blood exposure accidents accounted for 36.5%. On the social level, 42.2% reported disruptions in their family life. Thus, regular medical and psychological monitoring of night workers is fundamental.