Objective: To identify the determinants of the acceptability and skepticism of health workers about the anticovid-19 vaccination during the covid-19 pandemic in Senegal. Material and methods: This was a cross-sectional, descriptive study carried out among health personnel in the Sangalkam Health District from May 1 to May 15, 2021. A questionnaire was sent to the various health professional categories in the district. Anonymity and confidentiality were guaranteed. The data collected was entered and processed with Excel version 2013 software. Results: The participation rate was 82.5% (n = 140). Women represented 83.6% (n = 117). The average age of the respondents was 36.8 years. The average number of years of experience was 9.8 years. Nursing aides were more represented (23.6%). The vaccine rate was 87.1% (n = 122). The inactivated BIBP vaccine (Sinopharm) was the most administered (93.4%). The side effects were minor or even moderate and affected 30.3% of the vaccinees. On the other hand, 12.9% (n = 18) of the respondents were not vaccinated. The main determinants of vaccine acceptance were concern for protection (68%) and compliance with instructions from the hierarchy (29.5%). Mistrust of the information received (61.1%), fear of rumors (57.2%) and side effects (28.6%) were the main reasons for vaccine refusal. Conclusion: Awareness and communication are essential to remove barriers to anticovid-19 vaccination and increase acceptability.