Autoimmune diseases are marked by the presence of more or less specific autoantibodies for each of them. The detection and identification of these autoantibodies seem to be the pillars of the diagnosis. The aim of our study is to evaluate the ELISA technique compared to the immunodot, for the detection of extractable nuclear antigens antibodies Sm, SSA and SSB. This is a retrospective, comparative study of biological diagnostic techniques carried out over 6 months. 86 sera from patients tested by the immunodot for the detection of extractable nuclear antigens antibodies were analyzed by an ELISA test (AESKULISA) for the detection of anti-Sm, anti-SSA and anti-SSB antibodies. Sensitivities, specificities, PPVs, NPVs and the correlation index were calculated respectively for each of the three kits AESKULISA Sm (57.14%, 97.46%, 66.6%, 97.46%, 94%), AESKULISA SSA (53.84 %, 97.87%, 95.45%, 71.87%, 77.90%) and AESKULISA SSB (16.66%, 100%, 100%, 81.92%, 82.55%). Our study found low levels of sensitivity of the ELISA technique compared to the immunodot, which could be explained by the nature of the antigenic substrates, the coating procedures and the cut-off levels used by kits manufacturers. The immunodot appears to be more sensitive and more specific for the detection of anti-SSB antibodies and more sensitive for the detection of anti-SSA 52kDa antibodies. Indeed, a combination of two or more methods is to be recommended in order to optimize the relevance of the diagnostic test for the screening of anti-ENA antibodies.