Vitamin A deficiency is one of the most common and serious nutritional deficiencies in Burkina Faso. However, several ways to prevent and fight against these health risks exist, including the consumption of food provitamins A from a varied diet. The present study aims to assess the β-carotene content of three varieties of mangoes (Kent, Keitt and Springfield) producted and consumed in Burkina Faso. Twelve mangoe samples including four mangoes per sample were collected in twelve mango production fields from high production area in Burkina Faso. Their β-carotene contents were analyzed in triplicate using high performance liquid chromatography. The average contents expressed in micrograms of β-carotenes per hundred grams of mangoes are as follows: Kent (376,4 µg / 100g), Keitt (664,6 µg / 100g) and Springfield (471,9 µg / 100g). Based on the origin of studied mangoes varieties, it should also be noted that the varieties grown in the Banfora area have a higher β-carotene content (Kent (425,5 µg/100g), Keitt (773,5 µg/100g) and Springfield (534,6 µg/100g).) compared to those grown in others areas such as Bobo Dioulasso and Orodara.