The stem borer Eldana saccharina infestation level on Borotou-Koro sugar complex is very alarming. However, effective control methods are not yet very developed against this pest. In order to better understand and guide the search for a lasting solution, it is necessary to master the biology and ecology of this insect pest. It is in this context that this study was conducted at Borotou-Koro agricultural integrated unit. It aims to inventory the natural enemies of Eldana saccharina, in this case the parasitoids present on the sugarcane plots. To do this, borer larvae and pupae were collected from the sugarcane plots and reared in the laboratory. The results obtained indicate that three Lepidoptera species of stem borer are present on the Borotou-Koro sugar complex: Eldana saccharina (98.4%), Sesamia calamistis (1.53%) and Sesamia inferens (0.07%). The parasitism rate of E. saccharina larvae and pupae is low (3.29%). Six species of parasitoid auxiliaries of Eldana saccharina have been identified including a larval parasitoid (Sphaephoria sp), a larval-pupae parasitoid (Lycoriela auripila) and four pupae parasitoids (Trichopeza sp, Proctotrupes sp, Proconura nigripes, Psilochalcis sp). Fusarium spp has also been identified as a parasite of Eldana saccharina larvae. These six parasitoid species come from two families which are the Diptera estimated at 3.32% and the Hymenoptera which represents 96.68% of the percentage of parasitoids encountered. Thus, the Borotou-Koro sugar complex is full of several species of Eldana saccharina parasitoids on these sugarcane plots. Eldana saccharina larvae and pupae rate of parasitism is substantially identical for all the varieties of cane encountered. However, the parasitism rate of pupae varies greatly from one sector to another and is higher in rainfed conditions than in irrigated conditions.