Diabetes mellitus is a chronic metabolic disorder which is associated with hyperglycemia. It is caused by a derangement in the secretion or function of the endocrinal portion of the pancreas. The aim of the present study was to determine the blood glucose, MDA, amylase levels in 50 cases of type 2 Diabetes Mellitus and to compare and correlate these parameters with those 50 of age and sex matched healthy controls. Glucose and amylase concentrations were analyzed spectrophotometrically by kits in all patients with DM as well as in the control subjects but MDA concentrations was measured by manual methods The results of type 2 DM were compared with control group using One way ANOVA-test to compare parameters in different studied groups. The results show a significant elevation (P ? 0.05) in levels of glucose, MDA, amylase of type 2- diabetic patients in comparison healthy subjects.