This study aims to analyze the psychological well-being of trainee teachers in the professional master’s program at INJEPS and the organizational factors that influence the Psychological Well-Being at Work (BEPT) of these trainee teachers. Specifically, it was a question of evaluating the psychological well-being of trainee teachers in the professional master’s program at INJEPS. In order to achieve these objectives, a study was conducted within our target population. Thus, 63 trainee teachers in the professional master’s program at the National Institute of Youth, Physical Education and Sport responded to an electronic questionnaire. Data collection was done using an online questionnaire, developed using Google Docs. The results reveal that trainee teachers have a fairly satisfactory level of well-being but that one in five teachers has a low level of BEPT. The results of the linear regression analysis established that the managerial relationship, the availability of material resources, the school climate and professional skills have significant and positive effects on the psychological well-being at work of INJEPS professional master’s trainee teachers, while the workload and travel constraints at work have a negative and significant impact. These variables constitute positive factors whose growth contributes to the BEPT. In statistical terms, the variables, workload (β = - 0.063; Sig = 0.032 < 0.05) and travel constraints at work (β = - 0.067; Sig = 0.041 < 0.05) negatively and significantly affect psychological well-being at work.