Sesame (Sesamum indicum L.) is a vital oilseed crop extensively cultivated in tropical and subtropical regions. This study aimed to assess the agro-morphological performance and genetic variability of 11 sesame lines under the Sudano-Sahelian conditions of Burkina Faso. Conducted over three years at the INERA/Saria’s experimental station, 12 phenological and agronomic traits were evaluated using a randomized block design with three replicates. Results revealed significant differences among lines for most traits, including flowering time (DFLS), capsule length (HFCI), and yield components. Heritability values were high for parameters such as plant height at maturity (62.24%) and height of the first capsule insertion (79.31%), indicating their strong genetic influence. Yield variability was substantial, with the Wollega line achieving the highest mean yield of 1612.82 kg/ha. This analysis provides critical insights into the genetic potential and adaptation of sesame lines, laying the groundwork for future breeding programs to enhance productivity and resilience in semi-arid regions.