Due to the emergence of nanoscience and technology, metallic silver nanoparticles (AgNPs) are used as antimicrobial agents and are synthesized following various protocols. Several methods (Physical and Chemical) are used to synthesize nanoparticles, but biological methods are preferred due to their environmental friendliness, cleanliness, safety, cost-effectiveness, ease and efficiency for high productivity and purity. Green synthesis of nanoparticles (NPs) is a promising new tool in the field of bio-nanotechnology. Intra- or extracellular biosynthesis of NPs can be achieved by a wide range of biological entities, including bacteria, fungi, yeast, algae, actinomycetes and plant extracts. Biosynthesized NPs are characterized using a variety of techniques, including UV-vis spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infrared (FTIR) analysis and zeta potential analyses. NPs synthesized using the green approach can be used in the food industry, smart agriculture and wastewater treatment. They can be incorporated into various biotechnological fields as antimicrobial agents, antioxidants and phytopathogen control agents. This review will focus on the role of biosynthesized AgNPs for their antimicrobial application, leading to improved health, environment and prevention of infectious diseases.