Shea is a species native to the Sudanian-Sahelian zone. This species is confronted by climate change and human activity. Unfortunately, young plants do not have time to grow due to their very slow growth rate in their natural environment. An in vitro production study at the IPR/IFRA Laboratory of Agro-physio-genetics and Plant Biotechnology is underway for the improvement of this species. A completely randomized design was used. The plant material consisted of three provenances of leaf explants. The parameters observed were contamination, the rate of explants initiated, the rate of explants forming calluses, callus expression, weight, callus diameter in light and dark conditions, and callus regeneration. The STATITCF software was used for data analysis. The best disinfection rate was observed with the T3 test, with 2.5% contamination. The survival rate showed no significant difference (P=0.0934). However, a highly significant difference was found between the sources, with 75.35% for the Siby explants. For callus induction, a highly significant difference (P=0.0000) was observed. Regarding provenance, the analysis of variance showed no significant difference (P=0.9557). Different callus shapes and appearances were observed under conditions of light and continuous darkness Analysis showed a highly significant difference (P=0.0119) in callus survival rate between expression media. Under light culture conditions, the diameter and weight of the calluses showed no significant difference (P=0.3101). However, under continuous darkness, the analysis of variance revealed a highly significant difference (P=0.0018) of 1 cm and 1.39 g, respectively.