Cadmium (Cd) is so perilous pollutant for living things when the certain threshold concentrations exceeded. Barley can accumulate higher concentrations of Cd but show genotypic differences in Cd uptake, moreover, the response to Cd have not been clearly determined yet. Exogenous use of thiourea ameliorates the stress conditions. The objective of this work was to determine the alleviation of various cadmium levels (Cd 0, 100, 500 and 1000 and 1500