L-Lysine fermentation by Corynebacterium glutamicum obtained productivity improvements from D-glucose. Immobile Corynebacterium glutamicum on carrier brings many significant advantages for lysine production such as high reused ability, saving seed preparation stage and high adaptation ability of immobile cells to the fermentation environment. Simultaneously, immobile cells have a long phase for lysine excretion. L-Lysine fermentation kinetics by immobile cells is studied in the basic way to solve problems related to cell physiology. Thereby, optimization techniques are carried out more easily to determine the most suitable nutrient medium as well as fermentation conditions and result in obtaining highest yield L-Lysine. L-lysine concentration was improved to the level of 28.767
Nattokinase is able to hydrolyze fibri oriented fibers, reducing blood pressure to the arteries and heart valves. People use nattokinase enzyme through ingestion by natto food or functional food to dissolve blood clots. In fermentation by Bacillus subtilis natto, the bacterium can generate this enzyme. The optimized culture medium has 6 factors such as glucose, soybean peptone, K2HPO4, MgSO4, NaCl, CaCl2 and the rate of these in medium is identified by response furface methodology (RSM) and central composite design (CCD). The highest nattokinase yield was 69.3
Show abstract
Full Text
