This study aims to determine the impact of fortification of wheat flour by the undefatted seed of Citrullus lanatus (Cucurbitaceae) on blood biochemical parameters and biometry of organs. Thirty (30) rats were fed for 14 days with six diets. A control casein diet (RTC), a classic bread diet (RPC) and four diets fortified bread (RPFnd) where wheat flour was substituted for the rate 5% (RPFnd5); 10% (RPFnd10);15% (RPFnd15) and 20% (RPFnd20) by undefatted seed meal of Citrullus lanatus. After the experimental period, the animals were sacrificed and their blood is levied to assay metabolites. Their organs are also levied and weighed. The results indicate that glyceamia and uremia for the rat of RPFnd diet have undergone changes compared to those of rats fed by RTC and RPC diets. The fortification had no effect on other blood biochemical parameters. The animals of CBD diets had a ponderale increase kidney and stomach substitution rate of 15% and/or 20% compared to the rats of RTC diet. There was no change in other organs weight (p ≥ 0, 05) for all diets. However the rats of diet RPFnd20 had got light-colored livers. Histological studies are, nevertheless required to see if these changes have pathological consequences of these organs.