This study aimed to evaluate the effect of heterosis on chlorophyll and carotenoid content in a hybrid soybean. This study used two genotype of the elders, and two genotypes of F1 and F1R a hybrid varieties Petek x Jayawijaya. Petek and Jayawijaya were tolerant and sensitive to shade respectively. Observed variables were the content of chlorophyll, carotenoids, plant height, number of pods per plant, seed weight per plant and weight of 100 seeds. Heterobeltiosis value and the highest positive heterosis found in the character of pods per plant and pods per plant ranged contains 75-77% for mid parent and 41-45% for high parent, while the influence of the female elders are not present in the character of chlorophyll and carotenoid contents, plant height, number of pods per plant, seed weight per plant, weight of 100 seeds. The result of the research showed that there is no correlation between either chlorophyll or carotenoids content and other variables.